Hello Researchers
I am looking for the best and efficient in-vitro translation kit for my work.
My main objective is to get good results from the kit.
Promega, T7 30 extract for circular plasmid
In order to prepare the stock solution for enzyme assay, I need to verify that my NADPH salt is not oxidized. What precautions should I take during the preparation of the stock solution?
29 April 2023 2,375 0 View
To overcome this problem Polyether, polyester based, or some other technology will have great result in curing properties. Please help me in detail.
19 April 2023 2,721 0 View
Can anybody please share the Glycerol and Sucrose estimation protocol? I want to check the Glycerol and Sucrose concentration in the yeast culture. I really appreciate any help you can provide.
26 December 2022 7,982 4 View
Why is phenol-chloroform used in nucleic acid extraction and not any other solvent ? There are many solvents, but why only phenol-chloroform is used...
04 December 2022 4,185 2 View
Hello everyone I am looking for a PAGE gel protocol/recipe for RNA.
06 April 2022 2,347 2 View
I am working on the development of a PMMS model. To select the best-performing tools and models, several models are needed to be developed and validated. Can this be replaced by some optimization...
04 April 2022 1,929 5 View
Hello Researchers I need to measure or calculate milliarbitrary units (mAU) of RNAse? A total of 3.8 mAU of RNAse (Life Technologies) per μg of RNA was added to the formulations Can anybody please...
23 March 2022 494 1 View
Anyone please suggest the NABL lab for saffron quality testing in India
03 February 2022 7,447 0 View
Any international body that provide environment pollution data !!
03 January 2022 6,722 2 View
CIPS- Cross Sectionally augmented lm, Pesaran, and Shin unit root test) CADF- Cross Sectional augmented Dickey Fuller unit root test Both CIPS and CADF tests are performed under the null...
01 January 2022 7,362 0 View
I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...
10 August 2024 4,347 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,284 4 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View
My supposition or question: Is the 600 tetrahedron cell {3,3,5} polytope a four dimensional quasicrystal? I do think so, because its circumsphere is described by a radius of exactly the golden...
16 July 2024 4,653 0 View