You can try to read this, it may be useful

https://www.materials-talks.com/zetasizer-mrna-drug-delivery-review/

mRNA molecules cannot have a zeta potential.

I know that sounds very wrong to many people but here are the reasons:

1. mRNA is, in effect, an amphoteric polyelectrolyte comprised of nucleotides.

2. The ionizable moieties (-NRH and -COOH) are fixed spatially

3. The molecule will assume a configuration that is likely to permit the flow of surrounding liquid through it (semipermeable). This means there is no delineating plane of charge but, instead, a volume of fixed charged species.

Zeta potential assumes the following:

1. There is a clearly defined plane delineating the flow of surrounding fluid and the particle

2. For Smoluchowski/Huckel/Henry calculations, it is assumed that the particle is: (a) spherical, (b) impermeable to liquid and (c) all the charge is uniformly distribution across the surface of the impermeable sphere.

Hence, the concept of zeta potential does not apply to polyelectrolytes such as RNA and proteins. Instead, other theories relating electrophoretic mobility to charge (as opposed to potential) should be used.

You can learn more from my paper published last year: Article Determination of Protein Charge in Aqueous Solution Using El...

As I mention in the paper, molecules such as proteins and RNA form hydrophilic colloids, not hydrophobic. Hence, the usual concepts of stability (DLVO theory and the nonsense rule-of-thumb that ">30mV is stable") simply do not apply.

If you are wanting to measure the "zeta potential" of mRNA to relate it to stability, you will be disappointed.

Anifowoshe Abass The Malvern article is concerned with lipid nanoparticles containing mRNA, not mRNA itself. The measurement challenges and interpretation of data are very different (as per my answer above).

Anjaneyulu Dirisala

do you have any DLS data file you can show? The quality of the DLS data and repeatability will determine whether the hydrodynamic size of your sample is reliable. If you do not have the raw data, take a look at the z-average and PDI, and compare that to the size you expect. It may provide a first pass at how reasonable the diameters are.