Can anyone please provide me with PAGE gel recipe for RNA ?

More Abhilek Kumar Nautiyal's questions See All

How to confirm that NADPH is working fine or not oxidised?

In order to prepare the stock solution for enzyme assay, I need to verify that my NADPH salt is not oxidized. What precautions should I take during the preparation of the stock solution?

29 April 2023 2,375 0 View

Does the lower temperature and lower humidity effects the curing time of 2component solvent free adhesive for lamination. If yes then how to resolve?

To overcome this problem Polyether, polyester based, or some other technology will have great result in curing properties. Please help me in detail.

19 April 2023 2,721 0 View

Any protocol for Glycerol and Sucrose estimation?

Can anybody please share the Glycerol and Sucrose estimation protocol? I want to check the Glycerol and Sucrose concentration in the yeast culture. I really appreciate any help you can provide.

26 December 2022 7,982 4 View

Why is phenol-chloroform used in nucleic acid extraction and not any other solvent ?

Why is phenol-chloroform used in nucleic acid extraction and not any other solvent ? There are many solvents, but why only phenol-chloroform is used...

04 December 2022 4,185 2 View

Are there any optimization algorithms which can make a selection of the best-performing model process easy at the initial stages of model selection?

I am working on the development of a PMMS model. To select the best-performing tools and models, several models are needed to be developed and validated. Can this be replaced by some optimization...

04 April 2022 1,929 5 View

How to measure or calculate milliarbitrary units (mAU) of RNAse ?

Hello Researchers I need to measure or calculate milliarbitrary units (mAU) of RNAse? A total of 3.8 mAU of RNAse (Life Technologies) per μg of RNA was added to the formulations Can anybody please...

23 March 2022 494 1 View

Anyone please suggest the NABL lab for saffron quality testing in India other than IIIM Jammu?

Anyone please suggest the NABL lab for saffron quality testing in India

03 February 2022 7,447 0 View

How could I get the Nitrogen and Sulphur dioxide emissions freely available database?

Any international body that provide environment pollution data !!

03 January 2022 6,722 2 View

Which approach is considered to give more reliable results - CIPS or CADF for panel unit root test?

CIPS- Cross Sectionally augmented lm, Pesaran, and Shin unit root test) CADF- Cross Sectional augmented Dickey Fuller unit root test Both CIPS and CADF tests are performed under the null...

01 January 2022 7,362 0 View

What is the Zeta potential of mRNA?

Can anybody brief me on the zeta potential of mRNA?

26 December 2021 8,861 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View