I am adding ribosome binding site (GGAG) in primer to direct protein synthesis.Is this affect the protein synthesis.I am using pET28a vector?
In order to prepare the stock solution for enzyme assay, I need to verify that my NADPH salt is not oxidized. What precautions should I take during the preparation of the stock solution?
29 April 2023 2,375 0 View
To overcome this problem Polyether, polyester based, or some other technology will have great result in curing properties. Please help me in detail.
19 April 2023 2,721 0 View
Can anybody please share the Glycerol and Sucrose estimation protocol? I want to check the Glycerol and Sucrose concentration in the yeast culture. I really appreciate any help you can provide.
26 December 2022 7,982 4 View
Why is phenol-chloroform used in nucleic acid extraction and not any other solvent ? There are many solvents, but why only phenol-chloroform is used...
04 December 2022 4,185 2 View
Hello everyone I am looking for a PAGE gel protocol/recipe for RNA.
06 April 2022 2,347 2 View
I am working on the development of a PMMS model. To select the best-performing tools and models, several models are needed to be developed and validated. Can this be replaced by some optimization...
04 April 2022 1,929 5 View
Hello Researchers I need to measure or calculate milliarbitrary units (mAU) of RNAse? A total of 3.8 mAU of RNAse (Life Technologies) per μg of RNA was added to the formulations Can anybody please...
23 March 2022 494 1 View
Anyone please suggest the NABL lab for saffron quality testing in India
03 February 2022 7,447 0 View
Any international body that provide environment pollution data !!
03 January 2022 6,722 2 View
CIPS- Cross Sectionally augmented lm, Pesaran, and Shin unit root test) CADF- Cross Sectional augmented Dickey Fuller unit root test Both CIPS and CADF tests are performed under the null...
01 January 2022 7,362 0 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...
30 July 2024 9,824 2 View
I performed mmpbsa of protein-protein complex simulation on gromacs. My binding free energy is +166781. This complex is stable when experimentally produced in wet lab. In index.ndx I grouped...
19 July 2024 4,120 5 View
I have conducted virtual screening using Schrödinger on a database of 17,000 molecules. Unfortunately, I cannot use the system with the Schrödinger license at the moment. I am trying to find a way...
18 July 2024 2,881 4 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
Hi, I hope you are doing well, I am working to remove DNA from protein sample by using silica hydroxode magnetic beads. I used different binding buffers but I din't get my protein band in initial...
02 July 2024 5,502 4 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
02 July 2024 5,448 2 View