I am adding ribosome binding site (GGAG) in primer to direct protein synthesis.Is this affect the protein synthesis.I am using pET28a vector?
I want to make 5% DMSO solution for my substrate such as Fatty acids and naphthalene to dissolve.
04 April 2019 2,311 6 View
I want to extract my P450 reaction mixture and derivitized for GC analysis. I have read about BSTFA method, but i have no idea about the procedure. Please help me to understand the procedure.
26 January 2019 7,689 11 View
Its origin, antioxidant property?
23 January 2019 1,526 3 View
I have two independent domain cloned in E.coli and i want to fuse them both just to get a single domain by PCR. How to get this by PCR?
10 October 2018 1,718 4 View
facing problem in cloning.No colony in transformants plate.
19 August 2018 6,226 9 View
Dear all, I want only cytoplasmic content from the human cells to test my compounds. Does anyone knows how to do it or can share the protocol for doing it ?
29 June 2018 9,042 2 View
I have sintered Copper matrix composite which is reinforced by graphene, since graphene shows negative thermal expansion upto a certain temperature and copper shows positive thermal expansion...
04 February 2018 7,835 1 View
During XRD i found the peaks of CuAl2O4, CuMo2S3, AlB12 compounds and i want to know the effect of these compounds on tribological properties of Copper matrix composite during dry sliding. Plz...
16 November 2017 307 2 View
We have a pellet of size 30 mm diameter and 6 mm. thickness,it is a metal matrix composite, how i am supposed to measure the tensile strength of it, by which machine or facility or method it can...
18 July 2017 7,362 4 View
How to identify BL21 vs BL21(DE3) strain?
22 May 2017 4,209 4 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...
20 February 2021 4,332 4 View
1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...
20 February 2021 2,370 1 View
i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?
19 February 2021 2,352 5 View
Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...
16 February 2021 2,377 7 View
Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...
15 February 2021 4,353 3 View
Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...
15 February 2021 9,563 6 View
"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...
11 February 2021 6,628 9 View