Can I use G418 for selection of HEK 293 cell lines that already have a plasmid with kan and Hygro integrated?

I am doing a kill curve in HEK 293 cells with G418(0-1000ug/mL) and they don't die. They were previously transfected and selected with hygromycin and are integrated with a plasmid has also...

05 December 2020 3,232 3 View

Adaptability of frugal health care systems into developed markets - can someone share ideas on methodology for dissertation?

Hello! Hope everyone is doing alright during these turbulent and uncertain times. I am working on my masters dissertation (MSc International Management) and would like to answer the research...

05 August 2020 6,155 4 View

Do Transformational Leadership can be conducted qualitatively?

Most of the Transformational leadership study uses a quantitative method by doing a survey with an instrument that can be found. Is it possible to conduct it in a qualitative method, specifically...

16 July 2020 1,815 3 View

What is the best production evaluation method in an architectural design company?

I would like to know what is the most effective method to manage processes in an architecture company

29 April 2020 2,932 1 View

Dissertation topic for MSc International Management?

Hello, I am doing my masters in international management and looking for a research question related to start-up/ authenticity/ crowdfunding/ entrepreneurship & innovation to do my dissertation...

21 April 2020 5,222 23 View

What are the evidences of the AI's impact on the company's scalability?

How could we differentiate a usual scalability (organic or without AI technologies application) from an AI-impacted scalability of the enterprise?

27 August 2019 3,081 4 View

Plasmatic proteins solvent?

Can anyone suggest me a solvent to solubilize plasmatic proteins? I tried with formic acid, but was parcially disolved. Thanks

03 July 2019 10,030 2 View

How can I induce sickling consistently with sodium metabisulfite?

Hi all I'm trying to perform the procedure of sickling-induction in-vitro of peripheral red blood cells from sickle cell Townes mice, and I'm using 2% sodium metabisulfite. Unfortunately the...

20 January 2019 1,214 1 View

Hi, Is it normal when run PROCESS macro in SPSS for moderator (model 1) to get different results from when I run the linear regression in SPSS?

Could you please help me by giving me some advice on this? Thanks.

02 August 2018 2,012 2 View

Can I use at least 2% of fetal bovine serum in a transfection with Hiperfect?

I'm trying to transfect N1E-115 cells and the HiPerfect protocol advices not to add fetal bovine serum during the transfection phase. Unfortunately, my cells are dying without the FBS. Do you know...

23 October 2017 4,147 4 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

Acoustic simulation of Membrane?

I have 1 mm thick membrane. This membrane is perforated. I want to simulate this in Acoustic module of comsol. For this ,which type of module I can use in comsol.?

02 February 2021 2,505 6 View

Can i re-incubate a WB membrane with a higher solution of the previously used 1ry ab?

I've incubated my western-blot membrane with both primary and secondary antibodies and i just realized at the end that i've used a lower concentration of my primary antibody for my loading control...

27 January 2021 1,034 3 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View