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Good day colleagues/Seniors. How can one incorporate an armed conflict environment in the environmental uncertainty scale?Any literature to back that?

Urgent response needed

16 January 2024 6,577 0 View

How can I quantify Dengue Virus serotype 2?

Hi, I'm working with viral cultures, specifically with Dengue virus serotype 2. I need to quantify it by a plaque assay or TCID50. Does anyone have a protocol or has worked with this serotype...

10 December 2023 2,364 0 View

What are some design ideas for annealing Si samples (mounting adhesively or mechanically and heating up to 600°C)?

I need to anneal mm-scale samples made on Si substrates up to 600°C. To temporarily mount them, I've seen things like high temperature epoxies, tapes, and pastes as well as mechanical mounting...

10 November 2023 4,327 3 View

How to make cholesterol soluble in water?

Good morning, I want to dissolve cholesterol in Krebs-Henseleit buffer in tissue organ bath chamber where I place tracheal tissue. Krebs-H. buffer is buffer composed of water and salts similar to...

18 September 2023 4,774 0 View

Are there any tools for Gene ontology/Enrichment for Bacteria with unique locus tags and missing gene symbols (ID)?

I have a set of RNA data, 2 strains and three conditions. I did the mapping and Deseq analysis in geneious using our genome. But I ran into a problem that I'm struggling to resolve. I'm able to...

22 August 2023 7,575 1 View

Could someone please provide the procedure for trained Innate Immunity?

22 June 2023 9,337 3 View

SK-MEL-103 STR profile?

Hi all. Do you know the STR profile of SK-MEL-103 melanoma cell line? I guess it is not available from ATCC... Thank you.

16 May 2023 3,956 1 View

Is it possible to compare groups with different sample sizes while performing 2-way PERMANOVA?

I am dealing with unbalanced designs - comparing groups with different sample sizes, however I'm not sure I cancompare groups with different sample sizes while performing 2-way PERMANOVA.

30 January 2023 7,358 4 View

What is the best statistical analysis for FUCCI based cell system?

Hi all, I am having troubles choosing the best statistical analysis for my FUCCI (cell cycle indicator) experiment. Although I see that the majority of the publications do not use any...

27 October 2022 3,516 0 View

High Ct for GAPDH in FFPE samples?

Hello, I am working with FFPE tumor samples, and my Ct values are high (30) - I had 47,2 ng/uL RNA and used 300 ng for cDNA synthesis. I worked with Syber green and used 2 uL of cDNA in a final...

14 September 2022 3,177 1 View

I am carrying out an experiment on Trichoderma. How can I measure OD of the fungal growth after 7 days?

I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....

11 July 2024 6,251 1 View

¿Algún documento de consulta que presente claves dicotomicas para identificar especies de hongos microscopicos?

Hola a todos. Me gustaría saber si alguien conoce algún documento de consulta de claves dicotomicas - taxonómica para hongos microscópicos, especialmente que me pueda ayudar a determinar especies...

15 June 2024 6,693 0 View

Somebody know documents or books about free consultation that have cheats dichotomy for identify fungus microscopic ?

Hello I would like if you know some document for search cheats dichotomy - taxonomy for fungus microscopy, for example if you could help me to identify some kind of Beauveria, Penicillium y...

15 June 2024 1,289 0 View

Why is Trichoderma harzianum producing yellow pigment on rice broken at fermentation?

i have a species trichoderma harzianum. It is development in PDA media normally. But it is producing yellow pigment on rice broken at fermentation.

31 May 2024 9,260 0 View

How do i isolate and identify Trichoderma spp. from soil samples? what methods should i use?

i am working on Trichoderma spp.

24 March 2024 8,363 3 View

How to calculate enzyme loading for enzyme hydrolysis?

Good day, I hope everyone is well. I am looking for the correct method to calculate the required volume of enzyme to add to my flasks in order to perform enzyme hydrolysis in the conditions I am...

22 March 2024 3,382 0 View

Which is the best method to isolate Trichoderma in lab?

Which is the best method to isolate Trichoderma in lab? rice traps or PDA or a different method?

19 March 2024 8,154 1 View

What is the normal disc size of aspergillus and trichoderma specie?

I have to inoculate 6 discs of Trichoderma and aspergillus species in Potato dextrose broth in order to make the cell pellets of them .How do I measure or know the size of fungi discs .

13 March 2024 991 3 View

How the arbuscular abundance values can be the same in Irrigated and non Irrigated areas?

Arbuscular abundance values have the same values in Irrigated and non Irrigated areas using microbial inoculates such as; AMF, PGPR and Trichoderma

04 February 2024 8,488 0 View

How to grow trichoderma sporulation in flask & fermenter ?

Dear all I am working in fungal ( trichoderma ) batch cultivation with rice & soya flour after inculation culture grown fine but not sporulated . how to grow fungal sporulation in shake...

07 January 2024 5,091 3 View