I need to upload a sequence of a sequenced Trichoderma and I don´t know how.
What is species of Trichoderma, exactly...?
Is Trichoderma koningiopsis. I have ITS, rpb2 and tef partial sequences.
Urgent response needed
16 January 2024 6,577 0 View
Hi, I'm working with viral cultures, specifically with Dengue virus serotype 2. I need to quantify it by a plaque assay or TCID50. Does anyone have a protocol or has worked with this serotype...
10 December 2023 2,364 0 View
I need to anneal mm-scale samples made on Si substrates up to 600°C. To temporarily mount them, I've seen things like high temperature epoxies, tapes, and pastes as well as mechanical mounting...
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Good morning, I want to dissolve cholesterol in Krebs-Henseleit buffer in tissue organ bath chamber where I place tracheal tissue. Krebs-H. buffer is buffer composed of water and salts similar to...
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I have a set of RNA data, 2 strains and three conditions. I did the mapping and Deseq analysis in geneious using our genome. But I ran into a problem that I'm struggling to resolve. I'm able to...
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Could someone please provide the procedure for trained Innate Immunity?
22 June 2023 9,337 3 View
Hi all. Do you know the STR profile of SK-MEL-103 melanoma cell line? I guess it is not available from ATCC... Thank you.
16 May 2023 3,956 1 View
I am dealing with unbalanced designs - comparing groups with different sample sizes, however I'm not sure I cancompare groups with different sample sizes while performing 2-way PERMANOVA.
30 January 2023 7,358 4 View
Hi all, I am having troubles choosing the best statistical analysis for my FUCCI (cell cycle indicator) experiment. Although I see that the majority of the publications do not use any...
27 October 2022 3,516 0 View
Hello, I am working with FFPE tumor samples, and my Ct values are high (30) - I had 47,2 ng/uL RNA and used 300 ng for cDNA synthesis. I worked with Syber green and used 2 uL of cDNA in a final...
14 September 2022 3,177 1 View
I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....
11 July 2024 6,251 1 View
Hola a todos. Me gustaría saber si alguien conoce algún documento de consulta de claves dicotomicas - taxonómica para hongos microscópicos, especialmente que me pueda ayudar a determinar especies...
15 June 2024 6,693 0 View
Hello I would like if you know some document for search cheats dichotomy - taxonomy for fungus microscopy, for example if you could help me to identify some kind of Beauveria, Penicillium y...
15 June 2024 1,289 0 View
i have a species trichoderma harzianum. It is development in PDA media normally. But it is producing yellow pigment on rice broken at fermentation.
31 May 2024 9,260 0 View
i am working on Trichoderma spp.
24 March 2024 8,363 3 View
Good day, I hope everyone is well. I am looking for the correct method to calculate the required volume of enzyme to add to my flasks in order to perform enzyme hydrolysis in the conditions I am...
22 March 2024 3,382 0 View
Which is the best method to isolate Trichoderma in lab? rice traps or PDA or a different method?
19 March 2024 8,154 1 View
I have to inoculate 6 discs of Trichoderma and aspergillus species in Potato dextrose broth in order to make the cell pellets of them .How do I measure or know the size of fungi discs .
13 March 2024 991 3 View
Arbuscular abundance values have the same values in Irrigated and non Irrigated areas using microbial inoculates such as; AMF, PGPR and Trichoderma
04 February 2024 8,488 0 View
Dear all I am working in fungal ( trichoderma ) batch cultivation with rice & soya flour after inculation culture grown fine but not sporulated . how to grow fungal sporulation in shake...
07 January 2024 5,091 3 View