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Can I use G418 for selection of HEK 293 cell lines that already have a plasmid with kan and Hygro integrated?

I am doing a kill curve in HEK 293 cells with G418(0-1000ug/mL) and they don't die. They were previously transfected and selected with hygromycin and are integrated with a plasmid has also...

05 December 2020 3,232 3 View

Adaptability of frugal health care systems into developed markets - can someone share ideas on methodology for dissertation?

Hello! Hope everyone is doing alright during these turbulent and uncertain times. I am working on my masters dissertation (MSc International Management) and would like to answer the research...

05 August 2020 6,155 4 View

Do Transformational Leadership can be conducted qualitatively?

Most of the Transformational leadership study uses a quantitative method by doing a survey with an instrument that can be found. Is it possible to conduct it in a qualitative method, specifically...

16 July 2020 1,815 3 View

What is the best production evaluation method in an architectural design company?

I would like to know what is the most effective method to manage processes in an architecture company

29 April 2020 2,932 1 View

Dissertation topic for MSc International Management?

Hello, I am doing my masters in international management and looking for a research question related to start-up/ authenticity/ crowdfunding/ entrepreneurship & innovation to do my dissertation...

21 April 2020 5,222 23 View

What are the evidences of the AI's impact on the company's scalability?

How could we differentiate a usual scalability (organic or without AI technologies application) from an AI-impacted scalability of the enterprise?

27 August 2019 3,081 4 View

Plasmatic proteins solvent?

Can anyone suggest me a solvent to solubilize plasmatic proteins? I tried with formic acid, but was parcially disolved. Thanks

03 July 2019 10,030 2 View

How can I induce sickling consistently with sodium metabisulfite?

Hi all I'm trying to perform the procedure of sickling-induction in-vitro of peripheral red blood cells from sickle cell Townes mice, and I'm using 2% sodium metabisulfite. Unfortunately the...

20 January 2019 1,214 1 View

Hi, Is it normal when run PROCESS macro in SPSS for moderator (model 1) to get different results from when I run the linear regression in SPSS?

Could you please help me by giving me some advice on this? Thanks.

02 August 2018 2,012 2 View

Can I use at least 2% of fetal bovine serum in a transfection with Hiperfect?

I'm trying to transfect N1E-115 cells and the HiPerfect protocol advices not to add fetal bovine serum during the transfection phase. Unfortunately, my cells are dying without the FBS. Do you know...

23 October 2017 4,147 4 View

ITS primers for fungal characterization?

For example, I need to characterize Rhizoctonia and Trichoderma simultaneously. In literature they are giving ITS1 &ITS3 for Trichoderma and ITS2 &ITS4 for Rhizoctonia. In our lab there is...

19 January 2021 4,157 3 View

Is there an alternative for the enzyme mixture Driselase (from Basidomycetes) for Fusarium protoplast generation?

Dear all, is somebody familiar with an alternative to the enzyme mixture Driselase (from Basidomycetes) for Fusarium fujikuroi and/ or Fusarium graminearum protoplast generation? Were are using...

16 June 2020 5,865 2 View

Is there a catalogue of Trichoderma isolates? How are these isolates given names?

Hello everyone, I've been trying to find out how certain isolates are given a name and where to find a list of established names. e.g. there's T. asperellum T54, T. spirale T4, T. hazarnium T40...

10 April 2020 3,862 3 View

White fluffy growth at the base of Soyabean stem?

Good day, May you please assist me with the following: What is the white fluffy growth on the base of the soybeans stem(right at the bottom on the ground). It is very common, and plants are...

01 February 2020 6,339 5 View

Is it appropriate to inoculate a crop with arbuscular mycorrhizae and Trichoderma at the same time? Is it better to add both inocula separately?

Endophytic Trichoderma species are rather aggresive in their colonization of a substrate; I understand that they do it faster and more effectively than mycorrhizal fungi (e.g. Glomus spp.). It...

10 November 2019 9,263 7 View

Except CTAB method what is the best kit to extract Trichoderma spp. DNA?

I'd been tried CTAB method for this fungi many times but it gave my heavy RNA and unclear DNA bands, I don't know what is the problem and its solution.. so what is the best used kit for extraction?

10 November 2019 8,892 4 View

Trichoderma - literature on development of conidiophores/branchlets?

A question for Trichoderma specialists - has any research been done on the morphogenesis of conidiophores in Trichoderma and how that might relate to mycoparasitic structures? What I've noticed is...

08 September 2019 4,471 5 View

I wish to formulate trichoderma spore in talqa powder and liquid form. any one please suggest me ?

i wish to formulate trichoderma spore in talqa powder and liquid form. any one please suggest me ?

04 August 2019 4,211 3 View

I am trying to isolate protoplast form Leptosphaeria biglobosa with no luck. Please help?

Hello there, I am trying to isolate protoplast from Leptosphaeria biglobosa for a transfection experiment I am planning to do but so far after incubating my mycelia for 6 hours in 10ml of...

30 July 2019 4,179 3 View

I am trying to isolate protoplast form Leptosphaeria biglobosa with no luck. Please help?

Hello there, I am trying to isolate protoplast from Leptosphaeria biglobosa for a transfection experiment I am planning to do but so far after incubating my mycelia for 6 hours in 10ml of...

06 July 2019 9,163 4 View