Urgent response needed
I need to upload a sequence of a sequenced Trichoderma and I don´t know how.
20 May 2024 6,685 2 View
Hi, I'm working with viral cultures, specifically with Dengue virus serotype 2. I need to quantify it by a plaque assay or TCID50. Does anyone have a protocol or has worked with this serotype...
10 December 2023 2,364 0 View
I need to anneal mm-scale samples made on Si substrates up to 600°C. To temporarily mount them, I've seen things like high temperature epoxies, tapes, and pastes as well as mechanical mounting...
10 November 2023 4,327 3 View
Good morning, I want to dissolve cholesterol in Krebs-Henseleit buffer in tissue organ bath chamber where I place tracheal tissue. Krebs-H. buffer is buffer composed of water and salts similar to...
18 September 2023 4,774 0 View
I have a set of RNA data, 2 strains and three conditions. I did the mapping and Deseq analysis in geneious using our genome. But I ran into a problem that I'm struggling to resolve. I'm able to...
22 August 2023 7,575 1 View
Could someone please provide the procedure for trained Innate Immunity?
22 June 2023 9,337 3 View
Hi all. Do you know the STR profile of SK-MEL-103 melanoma cell line? I guess it is not available from ATCC... Thank you.
16 May 2023 3,956 1 View
I am dealing with unbalanced designs - comparing groups with different sample sizes, however I'm not sure I cancompare groups with different sample sizes while performing 2-way PERMANOVA.
30 January 2023 7,358 4 View
Hi all, I am having troubles choosing the best statistical analysis for my FUCCI (cell cycle indicator) experiment. Although I see that the majority of the publications do not use any...
27 October 2022 3,516 0 View
Hello, I am working with FFPE tumor samples, and my Ct values are high (30) - I had 47,2 ng/uL RNA and used 300 ng for cDNA synthesis. I worked with Syber green and used 2 uL of cDNA in a final...
14 September 2022 3,177 1 View
I am writing my thesis on the enculturation of Jamaican grief and bereavement practices that enhances their Christian faith and not diminish it.
23 July 2024 3,837 0 View
How do economic sanctions impact the foreign policy decisions of countries involved in geopolitical conflicts?
22 June 2024 7,923 4 View
1.How does it help? 2.Research questions
07 June 2024 5,234 4 View
I want how their chieftaincy is done and what brought the conflict
01 June 2024 5,315 0 View
considering perspectives such as social integration, conflict, and barriers to inclusion, as people are an organization's greatest asset
14 May 2024 7,614 0 View
Understanding the Impact of Regional Conflict in the Middle East on International Logistics and Supply Chain Management: Challenges, Strategies, and Resilience Mechanisms
03 May 2024 8,947 0 View
I conducted a Negative Binomial GLMM analysis on the dataset, and the results showed a significant difference with a P-value below 0.05 when I performed post hoc analyses, specifically pairwise...
01 May 2024 2,247 1 View
I am working with ecological count data where the raw data is divided into multiple sites among sampled years. So you have site 1,2,3...etc for year 1999, 2000, 2001, etc. Each year is within...
28 April 2024 8,675 0 View
Good day, colleagues. I do now write a thesis about the status of Jerusalem in the conflict between Israel and Palestine and my interest fell on the question of Jerusalem's special regime....
23 April 2024 6,202 4 View
In my testing of statistical significance by bootstrapping, p_value shows that there are no specific indirect effects, but confidence intervals bias corrected 2.5% - 97.5% does not contain the...
18 April 2024 7,913 7 View