How can I quantify Dengue Virus serotype 2?

Juliana Fernández Valenzuela @Juliana-Fernandez-Valenzuela

11 December 2023 0 2K Report

Hi,

I'm working with viral cultures, specifically with Dengue virus serotype 2. I need to quantify it by a plaque assay or TCID50. Does anyone have a protocol or has worked with this serotype before?

I am gratefull for any suggestions.

Badges
Science topic

Similar topics
Chemistry
Chemicals
Solvents

More Juliana Fernández Valenzuela's questions See All

¿Where to buy the Aspergillus oryzae NSAR1 strain?

Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...

11 July 2024 6,847 0 View

How to upload a sequence or create an account in Trichokey?

I need to upload a sequence of a sequenced Trichoderma and I don´t know how.

20 May 2024 6,685 2 View

Any bacteriolytic antibiotic for gram negative bacteria?

I need a bacteriolytic antibiotic for destroy only extracelullar gram negative bacteria Any suggestions?

16 May 2024 7,584 5 View

What is the optim temperature for protein denaturation in supernatants??

Hello! I´m developing a sandwich ELISA. My assay recognizes protein denaturalized very well, but there isn´t recognition when it is in its native form. So maybe if I heat the sample I could detect...

18 April 2024 5,671 3 View

Problems isolating human neutrophils using Percoll - Erythrocytes not pelleting?

Hello all, I present here a problem to see if someone has encountered it and can provide some clarity. We are trying to isolate neutrophils from patient blood in tubes EDTA. We are following the...

10 March 2024 4,155 3 View

Do my iPSCs have a normal morphology?

Hello everyone, I am starting to grow the SCTi003-A iPSC line for the first time and I am facing some drawbacks. Due to some problems with the first pass, I waited a bit longer than the 7-8 days...

22 February 2024 8,052 2 View

Transcriptomic data sets with Staurosporine?

Hi, Does anyone know about a public transcriptomic dataset (RNAseq, microarray) of Arabidopsis (or other plants) treated with Staurosporine? I checked in GEO or SRA, but I couldn't find any....

11 February 2024 8,621 0 View

Potassium Permanganate titration- more hydrogen peroxide detected than what is actually present in solution?

I have been performing titrations of a known concentration of hydrogen peroxide using 0.01N potassium permanganate and consistently requiring more potassium permanganate than what is estimated to...

07 February 2024 445 3 View

How can I resolve this convergence issue in the Abaqus CAE software?

I am conducting a sequential heat transfer-stress analysis for a composite wall consisting of steel plates and a concrete core, similar to concrete-filled tubes or CFTs. My analysis involves...

06 February 2024 1,524 6 View

Good day colleagues/Seniors. How can one incorporate an armed conflict environment in the environmental uncertainty scale?Any literature to back that?

Urgent response needed

16 January 2024 6,577 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,113 1 View

Dengue in Southern states in India?

There is rapid surge of dengue cases in Southern parts of India. However there are no cases till now in the setting I work in Northern India. Besides dengue surveillance and local preventive...

14 July 2024 2,935 2 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,481 3 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

Intracerebroventricular (ICV) AAV inj.?

I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...

12 July 2024 780 0 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,667 5 View

Does anyone know how to improve calcium-imaging fluorescence with GRIN lenses?

Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...

08 July 2024 5,176 2 View

Why do I have two different bands from the same virus after PCR?

I get two different bands from BCMV after PCR

07 July 2024 2,111 1 View

Problems to find a region of a Virus gene?

We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...

01 July 2024 7,640 3 View

I need this article, can anyone get it?

I need this complete article, could anyone help me? follow theme below. Chikungunya virus cell-to-cell transmission is mediated by intercellular extensions in vitro and in vivo.

20 June 2024 3,373 1 View