How can I compare two (or more) networks?

Suppose you have built a network based on gene expression or protein interaction. How can I compare the current network with that from KEGG? Is there any statistics or software? Best regards,

31 December 2014 115 3 View

How can I methylate or demethylate certain loci in human cell lines?

31 December 2014 2,586 3 View

Is there any recommended pipeline or procedure for Illumina Methylation 450K microarray analysis?

Is there any recommended pipeline or procedure for Illumina methylation 450K array analysis. and What we can do as a analyst when we obtain some methylation 450K array for some paired case and...

31 December 2014 4,793 9 View

Can anyone help to make a pathway analysis with Ingenuity Pathway Analysis?

31 December 2014 5,605 3 View

How can I get the secondary structure of pre-microRNA?

31 December 2014 3,834 4 View

Do you know of any R packages for analysis of case-control studies in genetic epidemiology?

As we know, case-control based genetic epidemiological studies has played important role in susceptibility factor identification. Now, would you mind suggesting some helpful R packages to do SNP...

31 December 2014 701 3 View

How can I do sample size estimation for GWAS study?

Usually, when we conducted candidate gene based association study, we need to estimate the sample size and the power. Is it necessary to do it when we conduct GWAS study? If yes, is there any...

31 December 2014 7,577 2 View

Is there any article on Relationship between DNA Methylation and Human Evolution?

DNA methylation was involved in the evolution of duplicate genes, gene expression, miRNA network stability. Question, Is there any great article to discuss The Relationship between DNA Methylation...

31 December 2014 3,815 1 View

Powerful pipeline for miRNA-seq analysis?

Which pipeline is a suitable one for miRNA-seq analysis? CAP-miRSeq, miRNAkey or others?

31 December 2014 114 4 View

How can I get the KEGG Pathway Network in mathematical form?

We can get the elements (genes) of each pathway of KEGG from KEGG website of UCSC database. However, how can we get the network relationship in the form of network for KEGG pathway as the...

31 December 2014 2,665 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View