Would anyone suggest me a vector(PiggyBac Transposon System) for stable miRNA overexpression in Lncap cells?

Mohammad Hassan Jafari @Mohammad_Jafari9

05 May 2018 1 5K Report

I want to overexpress a number of miRNAs in Lncap cells, I prefer to use a non-commercial and non-viral vector.

You could use a p.cDNA (go to addgene) type of vector and insert there the sequence coding your pre-miRNAs (+ a flanking region).

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