Does anybody have whole sequence information of binary vector pART27?

More Mohammad Hassan Jafari's questions See All

Would anyone suggest me a vector(PiggyBac Transposon System) for stable miRNA overexpression in Lncap cells?

I want to overexpress a number of miRNAs in Lncap cells, I prefer to use a non-commercial and non-viral vector.

04 May 2018 4,717 1 View

How can I express a premiRNA alongside GFP protein?

suppose I have a piggyback vector with this sequence: (5'-pB--promoter--chimeric intron--GFP-?-polyA--pB-3') now I want to express a premiRNA gene beside the gfp protein with same promoter and...

31 December 2017 7,457 7 View

Is there any online tool to compare two amino acid sequences folding?

is there any online tool to compare two amino acid sequences folding with ten amino acids conversion between them?

10 November 2014 5,181 0 View

What's the reason of antibiotic resistance when the plasmid appears to not exist?

I transformed agrobactrium (LBA 4404) with a binary vector with a kanamycin resistance gene. After transformation the colonies grow on the plate with kanamycin (final concentration 50ug/ml), But...

04 May 2013 6,813 15 View

Why the color of precipitated bacteria is black?

When I precipitate the bacteria for making the competent cells, the bacterial pellets are somewhat black. I use LB medium(10+10+5) & DH5-alpha for precipitation. I centrifuge them at 8000 rpm for...

02 March 2013 7,310 5 View

Problems cloning a 300 nucleotide fragment with XhoI and EcoRI restriction sites in pHannibal vector.

I want to clone a 300 nucleotide fragment in pHannibal vector with xhoi and EcoRI restriction sites. I designed two primers with these restriction sites in 5' ends of my...

01 February 2013 7,840 13 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View