Would anyone suggest me a vector(PiggyBac Transposon System) for stable miRNA overexpression in Lncap cells?

I want to overexpress a number of miRNAs in Lncap cells, I prefer to use a non-commercial and non-viral vector.

04 May 2018 4,717 1 View

How can I express a premiRNA alongside GFP protein?

suppose I have a piggyback vector with this sequence: (5'-pB--promoter--chimeric intron--GFP-?-polyA--pB-3') now I want to express a premiRNA gene beside the gfp protein with same promoter and...

31 December 2017 7,457 7 View

What's the reason of antibiotic resistance when the plasmid appears to not exist?

I transformed agrobactrium (LBA 4404) with a binary vector with a kanamycin resistance gene. After transformation the colonies grow on the plate with kanamycin (final concentration 50ug/ml), But...

04 May 2013 6,813 15 View

Why the color of precipitated bacteria is black?

When I precipitate the bacteria for making the competent cells, the bacterial pellets are somewhat black. I use LB medium(10+10+5) & DH5-alpha for precipitation. I centrifuge them at 8000 rpm for...

02 March 2013 7,310 5 View

Problems cloning a 300 nucleotide fragment with XhoI and EcoRI restriction sites in pHannibal vector.

I want to clone a 300 nucleotide fragment in pHannibal vector with xhoi and EcoRI restriction sites. I designed two primers with these restriction sites in 5' ends of my...

01 February 2013 7,840 13 View

Does anybody have whole sequence information of binary vector pART27?

Does anybody have or know where to find the whole sequence information of binary vector pART27 [which was constructed by Dr. Andrew Gleave(Plant Molecular Biology 20:1203-1207, 1992)]

01 February 2013 6,273 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View