When I precipitate the bacteria for making the competent cells, the bacterial pellets are somewhat black. I use LB medium(10+10+5) & DH5-alpha for precipitation. I centrifuge them at 8000 rpm for 10 minutes. I'm sure that they aren't contaminated because when I use them for transformation, they work properly.
Does anyone know what's the reason for this?
Dear Muhammad (Ali),
It is because not all of the bacteria in the culture are dead, only a percentage of the population are. It doesn't take a large number of competent cells to have a successful transformation.
Best.
Dear Mohammad,
The brownish - black pellet you are seeing are dead bacteria.
Best regards.
Dear Kecia, Thank you for the answer but I need more explanation. If it is so then why Mr. M. Jafari was successful in transformation? Can you please explain! Thanks
Dear Muhammad (Ali),
It is because not all of the bacteria in the culture are dead, only a percentage of the population are. It doesn't take a large number of competent cells to have a successful transformation.
Best.
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