Mohammad Hassan Jafari

7 Questions 15 Answers 0 Followers

Questions related from Mohammad Hassan Jafari

Would anyone suggest me a vector(PiggyBac Transposon System) for stable miRNA overexpression in Lncap cells?

I want to overexpress a number of miRNAs in Lncap cells, I prefer to use a non-commercial and non-viral vector.

05 May 2018 4,746 1 View

How can I express a premiRNA alongside GFP protein?

suppose I have a piggyback vector with this sequence: (5'-pB--promoter--chimeric intron--GFP-?-polyA--pB-3') now I want to express a premiRNA gene beside the gfp protein with same promoter and...

01 January 2018 7,492 7 View

Is there any online tool to compare two amino acid sequences folding?

is there any online tool to compare two amino acid sequences folding with ten amino acids conversion between them?

11 November 2014 5,214 0 View

What's the reason of antibiotic resistance when the plasmid appears to not exist?

I transformed agrobactrium (LBA 4404) with a binary vector with a kanamycin resistance gene. After transformation the colonies grow on the plate with kanamycin (final concentration 50ug/ml), But...

05 May 2013 6,847 15 View

Why the color of precipitated bacteria is black?

When I precipitate the bacteria for making the competent cells, the bacterial pellets are somewhat black. I use LB medium(10+10+5) & DH5-alpha for precipitation. I centrifuge them at 8000 rpm for...

03 March 2013 7,352 5 View

Problems cloning a 300 nucleotide fragment with XhoI and EcoRI restriction sites in pHannibal vector.

I want to clone a 300 nucleotide fragment in pHannibal vector with xhoi and EcoRI restriction sites. I designed two primers with these restriction sites in 5' ends of my...

02 February 2013 7,877 13 View

Does anybody have whole sequence information of binary vector pART27?

Does anybody have or know where to find the whole sequence information of binary vector pART27 [which was constructed by Dr. Andrew Gleave(Plant Molecular Biology 20:1203-1207, 1992)]

02 February 2013 6,304 6 View