Will NLRP3 be secreted in the cell lysate or the supernatant?

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Sandip Chakraborty

You can please follow the link below to obtain certain valuable informations:-

http://www.nature.com/ni/journal/v15/n8/full/ni.2919.html?WT.ec_id=NI-201408

You can also download pdf of the file attached herewith to have a better concept.

Divaker Choubey

NLRP3 is not normally "secreted" by cells. However, it can be released by myeloid cells. Further, NLRP3 is detected in exosomes that are released by certain cell types. Therefore, to detect the NLRP3 protein in exosomes, one needs to purify exosomes.