I am running an HMBC experiment on a Bruker 300 mHz instrument. I set up the experiment following the steps given in TopSpin guide book. Everything happens fine but when I try to open the spectrum at the end it gives the message "defined levels are outside of dataset range" How can I correct this? Thank you.
Keerthi Bandara Jayasundera, sometimes when you follow all steps provided in the user manual leads to this error mainly because your sample required a different data set than what is given in the manual. The commands could be the same, but the data and values would differ. So, ensure you have set the correct number of data points, spectral width, and transmitter frequency and that these parameters match the acquisition parameters used during the experiment. You can consult your instrument specialists; they might be able to help you out.
"Defined levels are outside of dataset range" is not an error message but an information that the defined levels of the 2D contour plot (to be defined with the command "edlev", see attachment) lie above the hjghest peak of your data map. Go down (either using the above-mentioned command or the buttons for height adjustment) to see if there is anything present in your spectrum. However, if I look at the projections shown in your screenshot, I presume you will find nothing but noise so perhaps something went wrong with your experiment setup or the acquisition process.
A quick way to get it into range is the command "levcalc". It calculates a set of levels based on LEV0 = lowest level , NLEV = number of levels and TOPLEV = highest level in %
Dear Keerthi,
...all correct what was said above.
Just to add: I am using in topspin the command/automation clev. Typing for example "clev 0.1 32" calculates and displays 32 logarithmic levels between 10% of the highest amplitude in the spectrum and the highest amplitude. To me this looks in most cases nice enough to work with....
Alfred
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