In the qualitative compound report obtained from HR-LCMS analysis of crude plant extracts, what is meant by Hits (DB)? Should we consider all the predicted compounds from the list for further studies?
That means the number of "Hits" for your compound in the database used. Basically, the number represents the possible compounds according to the thresholds and database used.
The HR-LCMS compound report provides a list of tentatively identified compounds with their "Hits (DB)" values, it is not recommended to consider all the predicted compounds for further studies without additional confirmation. The report should be used as a starting point for prioritizing compounds based on various parameters, but the final selection should be made judiciously considering the study objectives and available resources.
"Hits (DB)" refers to the compounds detected in the sample that match entries in a reference database (DB). These hits are essentially the compounds that the analysis software predicts to be present in the sample by comparing the acquired mass spectra with those in the database. Whether you should not consider all the predicted compounds from the list for further studies before precising it.