I need to concentrate some fatty acid methyl esters in a cell culture medium to separate and isolate them using Prep-HPLC. What type of solid phase extraction would be suitable to concentrate these molecules? Please share your experience. Thank you.
In the qualitative compound report obtained from HR-LCMS analysis of crude plant extracts, what is meant by Hits (DB)? Should we consider all the predicted compounds from the list for further studies?
30 June 2024 2,290 4 View
In my LC-MS experiment, I am utilizing an Agilent Polaris NH2 column to separate a mixture of glucose, fructose, and sucrose. I am using water and acetonitrile as A and B mobile phases...
26 March 2024 8,895 4 View
Or is it used only to analyze the modelled protein structures.
02 March 2024 6,662 2 View
Not able to view the downloaded model from ClusPro using PDBSum. Since both the docked proteins contain single A chain, PDBSum shows it as single image. Please give suggestions. Also tried to save...
18 January 2024 5,286 4 View
I am using a C30 column for carotenoid analysis. I bought the column recently and using it for the first time. When I use the column in LC-MS scan mode, I clearly see the UV-Vis peaks for my...
08 June 2023 2,357 2 View
I am analyzing Lycopene using LC-MS. I use a C18 column and my mobile phase is Methanol: MTBE = 95:5. I use an Agilent Ultivo QQQ mass spectrometer with APCI negative ion mode. I see the precursor...
07 May 2023 525 0 View
I am running an HMBC experiment on a Bruker 300 mHz instrument. I set up the experiment following the steps given in TopSpin guide book. Everything happens fine but when I try to open the spectrum...
09 March 2023 5,289 4 View
I used the following column for an HPLC separation; C18 4.6 x 250 mm, 5 um, 120 Ao. I used methanol and acetonitrile as mobile phases. Now I have to do LC-MS for the same samples. I am wondering...
19 December 2022 1,889 6 View
Hello, Can anyone please suggest the markers and protocol for FACS staining for macrophage from PBMC in human. Thank you.
09 March 2022 642 8 View
Scopus indexed quick publishing, genuine reviews, free publication, internationally reputed journals list.
02 February 2022 9,785 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.
28 July 2024 734 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
Sulfuric acid has changed color to yellow.
16 July 2024 6,964 0 View
I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...
15 July 2024 9,827 1 View
Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...
14 July 2024 7,475 1 View