When I left the Pseudomonas on medium like cetrimide agar, kings medium for more than 10 days. I found root like pattern on plates. Please someone explain the reason for this.
Please see the attachments for plates picture.
See the attached picture; the structure of the Pseudomonas aeruginosa colonies is similar to yours (with the root-like structure). Their bacteria is under stress. Looks like your plates are semi-dry. The picture was obtained from: http://www.bio-pro.de/standort/03483/index.html?lang=en&artikelid=/artikel/03579/index.html
See the paragraph: "Bacteria under stress" in the website.
Pseudomonas species is typically famous of making this pattern of growth due to motility of swarming type as it has flagella. So, after certain time period they form root like structures on plates.
I saw the same shape of Pseudomonas aeruginosa after leaving it for 2 weeks in incubator or in room temperature for about 2 months. I think the medium was dried and it is the shape of aggregation of strain beside the salts components in medium. The strain didn't form this shape due to stress because i cultured it after drying but i didn't obtain growth. I contrast the Bacillus strains was dried in my lab with the same shape but it grew after culturing again due t spore forming characteristics. So, all the data before confirm my thought.
Dear Partick,
I know that Pseudomonas is Gram negative bacteria. In my last answer I wrote (In contrast the Bacillus strains was dried in my lab with the same shape but it grew after culturing again due t spore forming characteristics. So, all the data before confirm my thought). I would like to compare Bacillus strain (spore forming bacteria) with Pseudomonas.
Thanks for your comment.
I have question regarding Cetrimide agar. We are doing soil testing for the presence of P.spp . in cetrimid medium, no colonies of Pseudomonas grow, While soil that has a high population of Pseudomonas,However, after 3 months of root-like colonies in petri seen
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