The untransduced T cells are produced by mock lentiviral transduction of human primary CD4+CD8+ T cells. These cells are subjected to comparable manipulations as CAR-T cells: activation, spinoculation (without lentivirus), and expansion. These T cells are meant to be negative controls in experiments using lentivirus-transduced primary CAR-T cells.
Why not to use Scramble or a vector without a construct? Why UTDs are used?
Mina Hosseini
Actually, since viral transduction may affect T cell function, you must always use an empty vector for the transduction of T cells and use it as negative control along with non-transduced T cells, and compare the results.
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