Hi everyone! I need to perform Western Blot but, unfortunately, my schedule allows me to be in the lab Monday and Wednesday (skipping Tuesday). Can I prepare gel and samples on Monday and run the gel on Wednesday? Will it affect the results?
Could you please share any research on interaction, mutual understanding, relationships, etc., between people with mental disorders? I am interested whether there can be situations when a mentally...
11 December 2022 9,413 10 View
Dear all, I need to synthetize a peptide for antibody production, since I had a hard time to purify one very stubborn protein. Please, help me to figure out the right strategy how synthesize a...
02 February 2022 3,881 5 View
Dear All , I was purifying the proteins, following ProBond™ Purification System protocol (denaturing conditions) and noticed that Ni-NTA resin turned into something like gel structure inside the...
02 December 2021 6,197 3 View
Dear All, I'm purifying some HIS tag protein in denaturing conditions and it seems that it does not elute from the beads efficiently (checked the gel).. Do you have any suggestions what is the...
24 October 2021 4,969 16 View
Copper and Cobalt can improve osteogenic differentiation of mesenchymal cells, but can it be somehow connected with hyaluronic acid?
16 April 2021 7,931 4 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,771 2 View
I asked the same respondents about how much luck, their own efforts and connections they need to achieve Goal # 1, # 2, # 3, # 4. (first group). The second group had the same questions and the...
09 August 2020 3,741 5 View
I do patch clamp (and Ca2+ imaging) and usually apply DAGs-DMSO in external buffer through the perfusion. Now I want to see if my ion channels will respond to cholesterol. I prepared...
05 August 2020 207 0 View
Hello! I am planning cross-cultural study in psychology. I've read various articles, but I can’t understand what are the requirements for translators? I’m at the stage one - translating the...
27 February 2020 2,518 11 View
Update I have a scale (12 items) I go to Analysis -> Scale -> Reliability analysis and get my Cronbach alpha (0,5) BUT 2 of my items are «inverse». If I recode this two items as it was not inverse...
23 February 2020 5,509 7 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,478 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,129 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,404 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 949 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,662 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...
12 July 2024 7,145 2 View