Mina Hosseini

5 Questions 8 Answers 0 Followers

Questions related from Mina Hosseini

Cell death in culture

Hi everyone, I've been recently culturing NIH3T3 cells. After a round of trypsinization and freezing, the thawed cells seem a bit unhealthy. I see random black clusters of cells approximately...

01 January 1970 5,867 7 View

What type of cell contamination is this?

Hi everyone, I've been recently culturing 4T1 cells. The cells looked happy and healthy after thawing for a few days (i changed the medium every other day); however, after trypsinization, they had...

01 January 1970 404 5 View

Concentration of retroviral particles with high-speed centrifugation

Hi everyone, Happy new year :) I am trying to produce gamma-retroviral vectors with an MSCV-based plasmid and the helper plasmid pCL-Eco via the HEK293T cell line. Since the produced viruses would...

01 January 1970 3,451 1 View

Mouse T cell death during isolation

Hi everyone, I have been trying to isolate CD3+ cells from mouse splenocytes using a nylon-wool column. Although the viability of splenocytes isolated from balb/c mice is almost 98%, the cells...

01 January 1970 8,851 0 View

Virus Packaging Problem with the Plat-E Cell Line

Hi everyone, I've been recently trying to produce ecotropic retroviral vectors using the Platinum-E (Plat-E) cell line thorugh calcium phosphate percipitation method. Although the transfection...

01 January 1970 7,735 2 View