Oleksandra Skorobohatko

5 Questions 3 Answers 0 Followers

Questions related from Oleksandra Skorobohatko

Can Copper and Cobalt ions improve hyaluronic acid uptake by mesenchymal stem cell line (C3H10T1/2)?

Copper and Cobalt can improve osteogenic differentiation of mesenchymal cells, but can it be somehow connected with hyaluronic acid?

17 April 2021 7,881 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

02 March 2021 8,591 2 View

After adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?

Yellow colour, before the reaction is stopped, is due to the formation of oxidized TMB, which isTMB(2+). The reaction is stopped by degrading HRP, but are there any further oxidations happening?...

14 December 2019 473 3 View

What are advantages and disadvantages of inducible promotors, namely of positive and negative regulation?

When we have a positive regulation the control is tight, so we have low background expression under non-induced conditions. Negative regulation can not be fully controlled, but as far as I...

26 January 2019 2,174 5 View

Why unstransduced T cells are used as negative control for CAR T cells?

The untransduced T cells are produced by mock lentiviral transduction of human primary CD4+CD8+ T cells. These cells are subjected to comparable manipulations as CAR-T cells: activation,...

01 January 1970 5,157 1 View