How to license a preprint?

I want to submit a research article on bioRxiv, but not sure of the license to use that will be compatible with most of the journals. CC-BY, CC-BY-NC, CC-BY-ND, CC-BY-NC-ND, CC0, or no reuse

10 November 2018 7,063 0 View

How to go with IMAC fractions eluted in 8M urea?

I am going to perform peptide purification for the first time in denaturing conditions with urea. This will be followed by HPLC for desalting. So my concern is where to store the fractions and for...

07 August 2017 4,619 1 View

What could be the reason for change in RT of the same sample?

I have run 300 uM of tryptophan in isocratic 50% methanol with 40 mM sodium citrate buffer pH 2.25, 400 uM SDS in a 40 min run at 35 degree and absorbance at 280 nm. During two reps the RT changed...

02 March 2017 4,025 8 View

What is the use of 254 nm wavelength in AKTA prime plus purification system?

I was just wondering why besides the 280 nm wavelength there is an option for 254 nm in the Akta Prime plus protein purification system. From a google search I only got to know that the absorbance...

10 November 2016 6,004 2 View

Do we have any agency like OSHA in India?

I was going through online lab safety training provided by NIH and could learn that me and many around me are working in unsafe conditions. Some labs are completely closed without a fume hood or...

05 June 2016 7,727 0 View

What to do with HPLC waste solvents?

03 April 2016 9,780 5 View

How to store IPTG induced cell pellet for whole cells activity analysis without losing activity?

Currently I am over-expressing the cloned gene in E.coli BL21(DE3) and harvest it and wash it with phosphate buffer. Then making a 20% cell suspension in 50 mM phosphate buffer pH 7.5 containing...

31 December 2015 5,677 4 View

How can I choose HPLC column for lantibiotic purification?

09 October 2015 2,273 4 View

What is the amount of DNAse I required to be added in lysozyme lysis of recombinant E. coli cells?

06 July 2015 5,920 6 View

What components need to be removed from an IMAC purified protein?

05 June 2015 3,446 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View