How to license a preprint?

I want to submit a research article on bioRxiv, but not sure of the license to use that will be compatible with most of the journals. CC-BY, CC-BY-NC, CC-BY-ND, CC-BY-NC-ND, CC0, or no reuse

10 November 2018 7,063 0 View

Why to use a colorimetric assay for DNA and protein estimation when these molecules give direct absorption at 260 and 280 nm?

08 September 2017 2,081 3 View

How to go with IMAC fractions eluted in 8M urea?

I am going to perform peptide purification for the first time in denaturing conditions with urea. This will be followed by HPLC for desalting. So my concern is where to store the fractions and for...

07 August 2017 4,619 1 View

What could be the reason for change in RT of the same sample?

I have run 300 uM of tryptophan in isocratic 50% methanol with 40 mM sodium citrate buffer pH 2.25, 400 uM SDS in a 40 min run at 35 degree and absorbance at 280 nm. During two reps the RT changed...

02 March 2017 4,025 8 View

What is the use of 254 nm wavelength in AKTA prime plus purification system?

I was just wondering why besides the 280 nm wavelength there is an option for 254 nm in the Akta Prime plus protein purification system. From a google search I only got to know that the absorbance...

10 November 2016 6,004 2 View

Do we have any agency like OSHA in India?

I was going through online lab safety training provided by NIH and could learn that me and many around me are working in unsafe conditions. Some labs are completely closed without a fume hood or...

05 June 2016 7,727 0 View

What to do with HPLC waste solvents?

03 April 2016 9,780 5 View

How to store IPTG induced cell pellet for whole cells activity analysis without losing activity?

Currently I am over-expressing the cloned gene in E.coli BL21(DE3) and harvest it and wash it with phosphate buffer. Then making a 20% cell suspension in 50 mM phosphate buffer pH 7.5 containing...

31 December 2015 5,677 4 View

What is the amount of DNAse I required to be added in lysozyme lysis of recombinant E. coli cells?

06 July 2015 5,920 6 View

What components need to be removed from an IMAC purified protein?

05 June 2015 3,446 8 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View