14 Questions 223 Answers 0 Followers
Questions related from Mangal Singh
I want to submit a research article on bioRxiv, but not sure of the license to use that will be compatible with most of the journals. CC-BY, CC-BY-NC, CC-BY-ND, CC-BY-NC-ND, CC0, or no reuse
11 November 2018 7,177 0 View
All the labs working on DNA and protein have UV/VIS spectrophotometer.
09 September 2017 2,200 3 View
I am going to perform peptide purification for the first time in denaturing conditions with urea. This will be followed by HPLC for desalting. So my concern is where to store the fractions and for...
08 August 2017 4,736 1 View
I have run 300 uM of tryptophan in isocratic 50% methanol with 40 mM sodium citrate buffer pH 2.25, 400 uM SDS in a 40 min run at 35 degree and absorbance at 280 nm. During two reps the RT changed...
03 March 2017 4,169 8 View
I was just wondering why besides the 280 nm wavelength there is an option for 254 nm in the Akta Prime plus protein purification system. From a google search I only got to know that the absorbance...
11 November 2016 6,379 2 View
I was going through online lab safety training provided by NIH and could learn that me and many around me are working in unsafe conditions. Some labs are completely closed without a fume hood or...
06 June 2016 7,834 0 View
We have 60 litres of organic solvents as HPLC waste collected in the solvent bottles, how can these be discarded?
04 April 2016 9,912 5 View
Currently I am over-expressing the cloned gene in E.coli BL21(DE3) and harvest it and wash it with phosphate buffer. Then making a 20% cell suspension in 50 mM phosphate buffer pH 7.5 containing...
01 January 2016 5,782 4 View
The lantibiotics that are currently purified in the literature, use all different columns like C4, C12 and C18. How to decide which one to use.
10 October 2015 2,389 4 View
I am breaking open my recombinant e.coli cells (20% solution in tris sucrose buffer) by lysozyme treatment. After the treatment, the solution turns very viscous. Then I centrifuge the solution at...
07 July 2015 6,032 6 View
My protein was present in 50 mM Sodium Phosphate, 500 mM NaCl and 10 mM Imidazole. It was loaded onto Ni-NTA and washed with same buffer but 20 mM imidazole. Then the protein was eluted using 50...
06 June 2015 3,559 8 View
I have to clone and overexpress a gene, upon analysis of the encoded protein sequence with Expasy ProtParam tool, it is showing a PII of >40, saying that the protein is unstable. How...
07 July 2014 5,138 4 View
I have packed a column with ~50 mL (height=28.5 cm) sephadex G-50 superfine, as required for the high resolution chromatography of a ~9.5 kDa peptide, in a BIORAD econo-column (d=1.5 cm, h=30 cm,...
01 January 1970 2,611 4 View
Its near the exclusion limit of 5 kDa of PD10
01 January 1970 8,101 4 View
