How to license a preprint?

I want to submit a research article on bioRxiv, but not sure of the license to use that will be compatible with most of the journals. CC-BY, CC-BY-NC, CC-BY-ND, CC-BY-NC-ND, CC0, or no reuse

10 November 2018 7,063 0 View

Why to use a colorimetric assay for DNA and protein estimation when these molecules give direct absorption at 260 and 280 nm?

08 September 2017 2,081 3 View

What could be the reason for change in RT of the same sample?

I have run 300 uM of tryptophan in isocratic 50% methanol with 40 mM sodium citrate buffer pH 2.25, 400 uM SDS in a 40 min run at 35 degree and absorbance at 280 nm. During two reps the RT changed...

02 March 2017 4,025 8 View

What is the use of 254 nm wavelength in AKTA prime plus purification system?

I was just wondering why besides the 280 nm wavelength there is an option for 254 nm in the Akta Prime plus protein purification system. From a google search I only got to know that the absorbance...

10 November 2016 6,004 2 View

Do we have any agency like OSHA in India?

I was going through online lab safety training provided by NIH and could learn that me and many around me are working in unsafe conditions. Some labs are completely closed without a fume hood or...

05 June 2016 7,727 0 View

What to do with HPLC waste solvents?

03 April 2016 9,780 5 View

How to store IPTG induced cell pellet for whole cells activity analysis without losing activity?

Currently I am over-expressing the cloned gene in E.coli BL21(DE3) and harvest it and wash it with phosphate buffer. Then making a 20% cell suspension in 50 mM phosphate buffer pH 7.5 containing...

31 December 2015 5,677 4 View

How can I choose HPLC column for lantibiotic purification?

09 October 2015 2,273 4 View

What is the amount of DNAse I required to be added in lysozyme lysis of recombinant E. coli cells?

06 July 2015 5,920 6 View

What components need to be removed from an IMAC purified protein?

05 June 2015 3,446 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View