Dear Researchers,
I made that medium by powder pack and added cell culture grade sodium bicarbonate. finally it converted to Complete DMEM. After some days a white color ball like object is formed inside the bottle. and also i separate that in to petridish for your viewing comfortable.
From the view of it, it looks like the mycelium of a fungus colony. Have a look at this pic of such a colony at https://commons.wikimedia.org/wiki/File:Oyster_mushroom_(Pleurotus_ostreatus)_mycelium_in_petri_dish_on_coffee_grounds.JPG
Best wishes!
It looks like a fungal contamination. Massive one. Did you watch it under the microscope?
To avoid such contamination, autoclave the media or filter through a 0.22 micron filter immediately after mixing it.
Massive fungal contamination. Throw everything away and start again. Then, as stated above, be fastidious with sterile technique, and filter sterilise before using. Good luck.
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