What kind of bubble this, which is form unattached cell lines and contain the movement particles with in bubble?

I am using HeLa cell for my studies and i am retriving the cells but it wont attach. the unattached cells are creating the extra bubble like structure and it contains the movement particle in...

10 November 2017 3,219 8 View

Why we use phenol red free medium (DMEM) for some experiment?

Is phenol red acting against cell lines?

06 July 2016 232 3 View

Why this white color ball-like structure is formed in my DMEN bottle?

Dear Researchers, I made that medium by powder pack and added cell culture grade sodium bicarbonate. finally it converted to Complete DMEM. After some days a white color ball like object is formed...

05 June 2016 5,004 4 View

How to do CAM assay?

Dear Researchers, How to make egg fertilization on petri dish for CAM model. Can any one give valuable suggestion for me?

05 June 2016 9,980 0 View

What kind of contamination could this be?

I maintain the liver cancer cell line for my studies. But i encounter some problems during sub culturing. What kind of contamination could this be?

05 June 2016 8,812 8 View

Can any one say. why it named as plasmid??

11 December 2015 6,165 0 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View