Why we use phenol red free medium (DMEM) for some experiment?

More Devan Umapathy's questions See All

What kind of bubble this, which is form unattached cell lines and contain the movement particles with in bubble?

I am using HeLa cell for my studies and i am retriving the cells but it wont attach. the unattached cells are creating the extra bubble like structure and it contains the movement particle in...

10 November 2017 3,321 8 View

Why this white color ball-like structure is formed in my DMEN bottle?

Dear Researchers, I made that medium by powder pack and added cell culture grade sodium bicarbonate. finally it converted to Complete DMEM. After some days a white color ball like object is formed...

05 June 2016 5,091 4 View

How to do CAM assay?

Dear Researchers, How to make egg fertilization on petri dish for CAM model. Can any one give valuable suggestion for me?

05 June 2016 10,105 0 View

What kind of contamination could this be?

I maintain the liver cancer cell line for my studies. But i encounter some problems during sub culturing. What kind of contamination could this be?

05 June 2016 8,901 8 View

Can any one say. why it named as plasmid??

what the reason of we called it as plasmid

11 December 2015 6,289 0 View

Those who are all having or working in HUVEC, can you are all connect with me?

cell culture work

10 November 2015 8,799 0 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

05 August 2024 5,311 0 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Subhash C. Juneja

Phenol red as some estrogenic activity and may intervene your experiment.

Pengyun Li

Phenol red is highly light-absorbent in the visible spectrum and can easily photo-sensitize cells, making them more susceptible to photo damage during fluorescence imaging.

Rose O'Reilly

How do you properly monitor media pH in culture without phenol red in your media? I'm switching to phenol-red free for a fluorescence assay.