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So we use Stemcell media, which has always been amazing, we coat all plasticware with collagen, and expand the primary nasal brushing from a 12 well plate, to a flask and then into the transwells....
06 June 2018 4,551 3 View
We have had a lot of success over the past few years growing and differentiating these cells from nasal and bronchial brushings, but since August, we can not even get them to stick to the plate...
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