I tried many times to separate the molecular size marker in the gel electrophoresis but no good results
life is full of the bad and sad news that may affect people life
04 May 2019 4,952 4 View
Boiling method is one of the simplest methods of DNA extraction, is it sufficient for the different applications of biotechnology
09 October 2018 705 0 View
DNA consist of series of regions coded for the whole genome.
05 June 2018 4,777 0 View
There is a process used for synthesis oligonucleotides on the glass slide
05 June 2018 6,207 1 View
Data for DNA sequencing that taking from organisms need to use computers to determine the differences between these organisms.
05 June 2018 4,974 0 View
every somatic cell in the body has basically the same DNA.
04 May 2018 1,441 0 View
chloroform is one of several chemical materials used in the extraction of DNA.
03 April 2018 3,370 2 View
Isoamyl alcohol is one of several chemical materials used in the extraction of DNA.
03 April 2018 8,552 2 View
one of the important steps in the extraction of DNA we use ethanol.
03 April 2018 3,720 0 View
In the process of DNA extraction, we use proteinase K.
02 March 2018 4,461 5 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...
02 March 2021 9,157 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View