chloroform is one of several chemical materials used in the extraction of DNA.
Chloroform is used in combination with phenol to remove protein from crude cell lysates. Chloroform helps phenol in the denaturation of proteins.
It facilitates in proper separation of aqueous and organic phase (clearly remove aqueous phase from organic phase with minimal cross contamination).
Chloroform is more proficient at denaturing proteins than either reagent is separated from everyone else. The phenol-chloroform blend lessens the parceling of poly(A)+ mRNA into the natural stage and decreases the development of insoluble RNAprotein edifices at the interphase. Phenol holds around 10-15% of the fluid stage, which brings about a comparable loss of RNA; chloroform keeps this maintenance of water and consequently enhances yields. Chloroform guarantees stage partition of the two fluids since chloroform is miscible with phenol and it has a higher thickness (1.47 g/cm3) than phenol; it powers a more keen division of the natural and watery stages in this manner aiding the evacuation of the fluid stage with negligible cross pollution from the natural stage
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