one of the important steps in the extraction of DNA we use ethanol.
life is full of the bad and sad news that may affect people life
04 May 2019 5,247 4 View
Boiling method is one of the simplest methods of DNA extraction, is it sufficient for the different applications of biotechnology
09 October 2018 771 0 View
DNA consist of series of regions coded for the whole genome.
05 June 2018 5,022 0 View
Data for DNA sequencing that taking from organisms need to use computers to determine the differences between these organisms.
05 June 2018 5,222 0 View
There is a process used for synthesis oligonucleotides on the glass slide
05 June 2018 6,462 1 View
every somatic cell in the body has basically the same DNA.
04 May 2018 1,537 0 View
Isoamyl alcohol is one of several chemical materials used in the extraction of DNA.
03 April 2018 8,853 2 View
chloroform is one of several chemical materials used in the extraction of DNA.
03 April 2018 3,713 2 View
In the process of DNA extraction, we use proteinase K.
02 March 2018 4,725 5 View
The DNA repair mechanism for photoreactivation
01 February 2018 8,627 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View