There is a process used for synthesis oligonucleotides on the glass slide
Solid-phase oligonucleotide synthesis. May additionally use "automated" and "CPG-based" (CPG stands for controlled pore glass, the most common base material for this procces).
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04 May 2019 4,952 4 View
Boiling method is one of the simplest methods of DNA extraction, is it sufficient for the different applications of biotechnology
09 October 2018 705 0 View
DNA consist of series of regions coded for the whole genome.
05 June 2018 4,777 0 View
Data for DNA sequencing that taking from organisms need to use computers to determine the differences between these organisms.
05 June 2018 4,974 0 View
every somatic cell in the body has basically the same DNA.
04 May 2018 1,441 0 View
chloroform is one of several chemical materials used in the extraction of DNA.
03 April 2018 3,370 2 View
Isoamyl alcohol is one of several chemical materials used in the extraction of DNA.
03 April 2018 8,552 2 View
one of the important steps in the extraction of DNA we use ethanol.
03 April 2018 3,720 0 View
In the process of DNA extraction, we use proteinase K.
02 March 2018 4,461 5 View
The DNA repair mechanism for photoreactivation
01 February 2018 8,369 1 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...
18 February 2021 2,769 4 View
Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...
17 February 2021 2,383 3 View
I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...
17 February 2021 8,986 2 View
I am doing research on the synthesis of paracetamol. I am preparing acetic anhydride solution with a few drops a sulfuric acid and wish to measure the pH of this solution. However my pH meter...
14 February 2021 8,699 3 View
I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...
11 February 2021 4,964 13 View
Hi everyone! I extracted RNA from urine pellet using phenol-chloroform method. The yield of RNA was 160ng/uL. 3uL of RNA was used for cDNA synthesis in 20 uL reaction using Go-Script kit....
05 February 2021 6,587 4 View
Hello, I am a synthetic polymer chemist. I would like to explore the simulation counterpart of my experimental work. Can anyone suggest a good software able to simulate polymerization reactions,...
04 February 2021 2,672 4 View
Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .
04 February 2021 5,614 3 View