Boiling method is one of the simplest methods of DNA extraction, is it sufficient for the different applications of biotechnology
life is full of the bad and sad news that may affect people life
04 May 2019 5,247 4 View
DNA consist of series of regions coded for the whole genome.
05 June 2018 5,022 0 View
Data for DNA sequencing that taking from organisms need to use computers to determine the differences between these organisms.
05 June 2018 5,222 0 View
There is a process used for synthesis oligonucleotides on the glass slide
05 June 2018 6,462 1 View
every somatic cell in the body has basically the same DNA.
04 May 2018 1,537 0 View
Isoamyl alcohol is one of several chemical materials used in the extraction of DNA.
03 April 2018 8,853 2 View
chloroform is one of several chemical materials used in the extraction of DNA.
03 April 2018 3,713 2 View
one of the important steps in the extraction of DNA we use ethanol.
03 April 2018 3,969 0 View
In the process of DNA extraction, we use proteinase K.
02 March 2018 4,725 5 View
The DNA repair mechanism for photoreactivation
01 February 2018 8,627 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View