What is the best and cheapest way to produce sub-micron (as small as possible) Mg powder?

I need very fine Mg powder (atleast less than 1 micrometer) for spark plasma sintering (SPS). would you please let me know which way you suggest to produce (or even prepare or buy) sub-micron...

06 July 2018 1,867 6 View

What is the best method to measure the energy of grain boundaries and grains?

For Mg based materials, some materials can be etched using picric acid and then the grain boundaries appears clearly, but for some of them like pure Mg and Ca containing alloy, grain boudnaries...

08 September 2017 8,806 4 View

How to distinguish phases or phase orientations using SAED (TEM)?

Using SAED we would have some bright points reflecting the orientation of a specific phase? Would you please let me know how I can analyze them and get to know the related phase? Thank you!

06 July 2017 5,284 5 View

Fraction of an Orientation Using EBSD!

Would you please let me know how I can measure the fraction (volume or area) of different orientations using EBSD?

05 June 2017 2,701 5 View

Is it possible to achieve exact and fixed potential value in SKPFM (AFM) by a Cypher AFM?

Hi, I used a cypher AFM with SKPFM (SKPM) technique to measure the surface volta-potential distribution for Mg alloys. But for a bare surface which has just recently been polished, I can not find...

04 May 2017 8,174 6 View

How can I measure the area fraction of grains (distinguished by a pole figure (texture) in OIM (EBSD) software?

Hi, I am wondered to know how I can measure the area fraction of each color in OIM software which is utilized for EBSD images? for example I want to measure the area fraction of red colors in an...

09 October 2016 8,472 2 View

What is the best method to measure dislocation density?

31 December 2015 1,762 18 View

How can one obtain the fraction of specific planes using texture analysis by XRD (X'pert Pro)?

For a Mg alloy, I analyzed the texture by X'Pert Pro, from two samples of extrusion and extrusion followed by forging. Can some of tell me how I can compare the fraction of different planes in...

09 October 2015 9,319 1 View

How can I find out the atomic position of a crystal with known space group(using http://www.cryst.ehu.es/ website)?

In the following link, the atomic position of different space groups has been...

11 December 2014 8,734 15 View

How to draw a hexagonal crystal in Materials studio (CASTEP)?

I am going to draw a hexagonal crystal for pure Mg, MgCl2 and solid solution mg phase with Materials studio. But I found some problem in drawing the crystal. For example for the case of Mg2Ca, I...

11 December 2014 8,312 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View