How to define the sink temperature and film coefficient?

Hi, everyone. I am considering the heat conviction during compacting simulation by Abaqus. I was wondering that how to define the sink temperature and film coefficient? Is there any references...

21 February 2021 6,197 1 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View

How to define thermal failure in Abaqus?

Hi, everyone, I am simulate the impacting CFRP model, I want to know how to define thermal failure during the impact process because epoxy degraded owing to thermal effects. Thank you so much for...

25 December 2020 7,424 3 View

How to define thermal conductance in Abqsus?

25 December 2020 3,596 1 View

ESA Eureca spacecraft Solar Array Arcing demage, What is the cause of the problem?

We see a photos of solar array damage suffered by the ESA Eureca spacecraft that was recovered by the Space Shuttle. photo shows a sustained arc site on its solar arrays. In this case, the...

23 December 2020 2,512 2 View

Micro-scale unit in Abaqus?

Micro-scale unit in Abaqus. Hi everyone, I am working on micro-scale simulations, the length of the model is micrometers. So, some information is provided on the internet, but some part is...

10 December 2020 8,448 3 View

How to reduce background noise when testing cholesterol level in rat serum?

l've got rat serum samples with different background (from pink to yellow) and l'm trying to test total cholesterol, HDL and triglyceride in mice serum. l'm worried that the background noise...

14 November 2020 6,628 3 View

Is Uniform Convergence property contradictory to No Free Lunch Theorem?

In Statistical Learning Theory, Uniform convergence means for a hypothesis class H, given m(\delta, \epsilon), for arbitrary distribution over domain (X, Y), with probability $1- \delta$, the...

03 November 2020 7,732 5 View

I put a sodium chloride which is less than solubility chart but it doesn't melt. can you tell me what the problem is?

24 September 2020 224 3 View

How to recognize and verify the crystallization occur or not?

Hi, everyone My feedstock powder is water atomized Sn with a diameter range from 10-100 um. After the cold spray processing, the EBSD maps can be obtained. Because my smallest powder is 10um,...

07 September 2020 7,766 4 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View