Which mitochondrial protein is most suitable as a comparison for expression of mitochondrial membrane proteins?

More Junhui Sun's questions See All

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Why neutralization is necessary in the method for the determination of methylglyoxal?

In most colorimetric determinations of methylglyoxal, there is neutralization using saturated potassium carbonate, and perchloric acid is added to the final reaction. I would like to understand...

23 June 2024 2,074 0 View

How to choose polymerization temperature from initiator half life?

For different initiators having different half life at different temperature, which half life is more practical for polymerization temperature control ? such as ten minutes, or 30 minutes half...

08 June 2024 3,104 6 View

Plasmid DNA transient transfection reproducibility using liposome ?

Hi, I'm trying to find a pDNA transient transfection carrier for my MDA-MB-231. I'm using either liposome or lipopolyplex, but the transfection variability between experiments are too great. I can...

28 April 2024 1,189 2 View

Can Autodock vina deal with protonated amines?

If so, do the input ligands need to be protonated with SMILES strings?

09 April 2024 5,547 1 View

Why the grain size refinement is not obvious after stirring friction machining of AZ31 magnesium alloy in rolled state?

I performed multiple passes of stir friction machining of AZ31 magnesium alloy (average grain size 12μm) under different cooling media. The results show that the average grain size can only be...

07 April 2024 6,848 0 View

How to calculate Native and Denatured GFP protein's Daltons?

Hi, I saw a picture on a web (https://www.chegg.com/homework-help/questions-and-answers/9-calculated-molecular-weight-native-gfp-denatured-gfp-native-denatured-proteins-differ-mo-q53517323). It...

01 April 2024 6,835 3 View

Code for PCA ?

Part of PCA code, I'm confusing about why need to decompose twice. for s=1:4 %Quadratic variation for each asset Var{s}=diag(diag(X{s}'*X{s})); %Quadradic correlation matrix...

01 April 2024 1,359 0 View

What does DAMASK runtime interrupt with the error "max number of cut back exceeded, terminating" mean?

What does DAMASK runtime interrupt with the error "max number of cut back exceeded, terminating" mean, and how do I fix this problem?

08 March 2024 7,943 4 View

Could you please delete the account?

Jianwei Sun PhD Moffitt Cancer Center United States

06 March 2024 5,099 1 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 966 3 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,292 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,623 1 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM images?

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?

25 July 2024 5,482 2 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View