why the RNA is very instable and because of its OH group is very degradable and the lncRNA is very stable and can inhibit or active the gene for rest of the life?
I want to plot the roc curve for real time data(5 genes). does with the roc curve can show which of these genes has more effect and more importance than others after drug treatment?
29 August 2019 4,151 2 View
The bacteria i'm working on is pretty resistant to my drug and so only the first well ( I'm using the 96 well micro-dilution method) doesn't have any turbidity and I've read that I shouldn't use...
22 October 2018 7,962 3 View
I have a model for electrophoresis of a particle,I need to update boundary condition in each time step. Actually I calculate boundary condition (velocity of wall) from dependent variables that...
19 January 2018 3,509 4 View
I want to model forces acting on single chared collide in nanochannel with charged walls. electrical potential diffrence set along the nanochannel and cause electroosmosis flow in channel. main...
16 December 2017 9,938 1 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...
26 February 2021 4,026 5 View
Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...
26 February 2021 1,100 5 View
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 8,618 3 View
I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...
24 February 2021 974 1 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View