why the RNA is very instable and because of its OH group is very degradable and the lncRNA is very stable and can inhibit or active the gene for rest of the life?
Hello. I have cultivated the species of dermatophytes in the special culture medium of SCC. Unfortunately, the rate of species growth is very slow. Does anyone have any experience in this field,...
26 May 2024 4,552 3 View
Are there any documents determining the number of processors and the cores per processor in SATA and NVMe SSD controllers? If not, is there a method for estimating them?
12 January 2021 1,556 3 View
I want to plot the roc curve for real time data(5 genes). does with the roc curve can show which of these genes has more effect and more importance than others after drug treatment?
29 August 2019 4,234 2 View
Having been stated in the literature, SSDs adopt dynamic mapping policies to map logical addresses to physical addresses in the back-end. Thus, the sequential logical addresses may be mapped to...
08 May 2019 9,435 0 View
Hello, I performed docking for my antibody-antigen complex using Z-dock. Is there any software that I can use to analyse the result of my docking by telling me the exact interacting residue and...
23 February 2019 6,014 3 View
I`m modeling a helmeted head impact to a steel anvil. I used MAT 126 to model the foam of the helmet and it seems like E (the elastic modulus for the compacted honeycomb material) is causing the...
22 November 2018 660 3 View
The bacteria i'm working on is pretty resistant to my drug and so only the first well ( I'm using the 96 well micro-dilution method) doesn't have any turbidity and I've read that I shouldn't use...
22 October 2018 8,049 3 View
I have a model for electrophoresis of a particle,I need to update boundary condition in each time step. Actually I calculate boundary condition (velocity of wall) from dependent variables that...
19 January 2018 3,614 4 View
For the first time, the Jurket cells were grown well, but in the following days it had no growth . What could be the reason?
11 January 2018 7,197 1 View
I want to model forces acting on single chared collide in nanochannel with charged walls. electrical potential diffrence set along the nanochannel and cause electroosmosis flow in channel. main...
16 December 2017 10,043 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View