Why is there no DNA in a sample when I crashed the mycelium cells by liquid nitrogen and stored it until extraction?

More Mohammad Ibrahim's questions See All

Restriction enzymes with genomic DNA?

Hello, Is there anyone has any experience with using restriction enzymes with DNA before doing PCR? in case having a pure extracted DNA

07 August 2018 9,343 4 View

Can using the same rapd primers for fungi and plant ?

Can using the same primers to detect the variation in plant and fungi by RAPD PCR ?

07 August 2017 7,667 7 View

Aflatoxin genes I isolate ten A. flavus all of them gaves positive in Aflatoxin producing. while in PCR only 2 gene (omt and nor)?

I isolate ten A. flavus all of them gaves positive in Aflatoxin producing. while in PCR only 2 gene (omt and nor) were detected . no bands for apa and ver genes, can the fungus produce Aflatoxin...

04 May 2015 2,704 1 View

Can I detect the mtDNA in total genomic DNA without specific producer for mtDNA extraction?

09 October 2014 6,198 6 View

Can I detect the chloroplast DNA in total genomic DNA without specific producer for chloroplast DNA extraction?

09 October 2014 1,026 2 View

PCR Troubleshooting

Hi, have anyone have an electronic book about PCR Troubleshooting?

07 August 2014 2,568 2 View

What is the annealing temperature suitable for the PCR reaction?

I have primers, but the problem is they have different Tm with a big range. The forward is 70 the reverse is 63.

04 May 2014 6,907 23 View

What types of RNA should I extract for reverse transcriptase PCR, miRNA or large RNA (Total RNA)?

03 April 2014 3,256 8 View

Can anyone explain this "plant contains genes that homologous in structure and function to genes of MT in animal and fungi"?

Does this mean they have the same genes? If they are homologous, can I use the same PCR primers?

03 April 2014 5,819 3 View

Can I use the same PCR primers with different DNA organisms?

Sometimes when I am looking for some microorganisms primer genes in the web I found some of them, but have been used with different DNA organisms, can I use the same primers with different organisms?

02 March 2014 3,035 12 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Selvaraj Jagannathan

Dear Khalil during the storage of mycellium have you added cryoptotectent (DMSO) with on the liquid nitrogen, because of that .

Mohammad Ibrahim

Thanks, but do think that some cell components can degrade the DNA or there is some effect of water on DNA if stored before doing the extraction?

Ayat AL-Laaeiby

You can keep your mycelium on deep freeze (-80)C and crash in the same day when you start extraction of DNA

Ravinder Kumar

As Ayat told that you have to store the mycelium at -80 c and use all the things autocleved because there may be some DNAase which can cause degradation.