Why is there no DNA in a sample when I crashed the mycelium cells by liquid nitrogen and stored it until extraction?

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Selvaraj Jagannathan

Dear Khalil during the storage of mycellium have you added cryoptotectent (DMSO) with on the liquid nitrogen, because of that .

Mohammad Ibrahim

Thanks, but do think that some cell components can degrade the DNA or there is some effect of water on DNA if stored before doing the extraction?

Ayat AL-Laaeiby

You can keep your mycelium on deep freeze (-80)C and crash in the same day when you start extraction of DNA

Ravinder Kumar

As Ayat told that you have to store the mycelium at -80 c and use all the things autocleved because there may be some DNAase which can cause degradation.