18 Questions 52 Answers 0 Followers
Questions related from Ayat AL-Laaeiby
I am thinking about conservative site PCR product. When PCR products were amplified by universal primer ITS 1and ITS4 region of rDNA, the products length differ among Candida species. Although the...
05 May 2019 1,516 4 View
Hi I have new sequence and I want to download in the NCBI website and get reference number. Does anybody can share the steps of this process? Regards Ayat
07 July 2018 7,927 0 View
I got some minus value in the my results, I repeated the experiment three time, and get same values. Does anybody could advice me that how can I treated them statistically? Thank you
05 May 2017 2,515 11 View
I want to count killing percentage by using MTT assay. I incubated spores with macrophage in time course and used MTT dye for overnight, dye concentration 0.1 mg/ml. My question is that I want to...
08 August 2016 7,741 2 View
Dear all, I am trying to count alive spore from dead one after incubation with macrophage by using flow cytometer, Does anybody has an idea, how can I select suitable label to my spores and...
07 July 2016 10,054 5 View
I transformed my strain with TOXA:GFP and trying to confirm the insertion by using southern blot technique. I can see the fluorescent under the microscope but it does not work with ELISA or...
04 April 2016 8,519 2 View
Does anyone has PCB1530 plasmid sequence or at least has the multiple cloning site? Thank you
01 January 2016 3,233 4 View
I read about DNA repair after exposure to UV light, photoreactivity is one of the mechanisms of repair. This mechanism is performed in the presence of light. I am wondering, if somebody exposure...
11 November 2015 9,330 2 View
Does anyone could explain the mechanism of DNA insert after cloning to a filamentous fungus and what is the fate of plasmid (which carry the insert)? Regards
09 September 2015 6,846 4 View
I read in all paper they use UV light with a wave length 254 nm with bacteria and fungi. My question is why 254 nm exactly? Regards
09 September 2015 3,878 6 View
I am trying to prepare spore suspension from 10^5 to 10^7 and inject 20 microliters to Galleria: 1- Spin 1ml of spore suspension to collect spores2- Re-suspend spores with 20 microliters My...
10 October 2014 8,354 7 View
I want to calculate my primer concentration to be able use the Tm calculator on New England Bio lab. I have primers and I need to amplify by using Phusion High-Fidelity DNA polymerase but I do not...
06 June 2014 2,935 9 View
Why did we use complementation inspite of how we examine how many copies of the gene mutated by southern blot?
05 May 2014 7,329 1 View
I want to design a primer to amplify a specific region by using Primer3 online. My problem is that I can not get primers to amplify all my fragment. So, could you help me to know how can I amplify...
05 May 2014 9,091 2 View
DIG southern blot
04 April 2014 2,179 13 View
I am confused about selecting of enzyme for a Southern Blot and where exactly to cut it. I am working with knock out and used hygromycin selectable marker.
02 February 2014 1,575 1 View
And what is the maximum range of annealing temperature?
12 December 2013 8,705 7 View
I need fill calculate formula that I can used it with other primers
10 October 2013 3,529 5 View
