Can using the same primers to detect the variation in plant and fungi by RAPD PCR ?
Why would you try the same primer sets in unrelated organisms? RAPD primers are usually used to quickly type different strains of the same species.
https://www.ncbi.nlm.nih.gov/probe/docs/techrapd/
In short, no.
Thanks for your reply katie, I meant can I use the same set of RAPD primers which used to detect variations in same species of fungi, can I use them to detect variation in same species of plant ?
Hi, I prefer to use a primer from a previous study, since some rapid primer is either for specific identification and other for differences. Although you'll usually get a bans but they 'll not be in the position where for detect the similarity among your species
Thank you Abdulazees Parveez Ahamed, I also expected that as the rapd primers are not specific and dont depending on the specific Template sequences when they designed.
Hello
Yes, you can. The major advantage of RAPD includes that, it does not require pre-sequencing of DNA. Also, in an examinarion, the PCR product enabled to distinguish the 11 plant species even with the use of a single primer.
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