Ravinder Kumar

8 Questions 43 Answers 0 Followers

Questions related from Ravinder Kumar

How to separate two close peak and peak tailing in HPLC?

I am using HPLC for saponin estimation in plant samples. but the problem with separation of the close peaks. the HPLC conditions are.. 1. HPLC: Agilent 1260 infinity with DAD detector.2 2....

08 August 2017 3,621 23 View

Which type of saponin is found in Aloe vera?

i want to estimate the saponin content in crude extract of aloe vera using HPLC. which standard of saponin should i use to estimate the saponin or which type of saponin is found in Aloe vera?

03 March 2016 3,791 3 View

Which is the best method for estimation of total Saponin from Aloe vera Using HPLC?

Suggest methos of total saponin estimation from Aloe vera. Also suggest the extration method and parameter of HPLC.

08 August 2015 9,010 7 View

Which solutions/chemicals should be used to remove the polyacrylamide gel from glass plates?

I am using Biorad sequi gen sequencing gel apparatus for AFLP. In which one a plate is treated with Bind silane. After staining this is very difficult to remove the gel from the glass plate....

06 June 2014 4,993 4 View

How do I design the SSR markers from NGS data?

We have sequenced the plant transcriptome using next generation sequencing. Now we have to Design SSR markers from this data. Please suggest how to design these markers.

04 April 2014 1,635 5 View

Can someone suggest the best suited DNA isolation method for many Fungal strains?

Seeking a suggestion of any DNA isolation method for different fungal strains. Some methods are working for particular strains. But I want a method which works for different strains and also best...

08 August 2013 3,374 1 View

Can someone suggest a HPLC Method for the extraction and estimation of saponin from plants?

Please suggest HPLC method for estimation of saponin. Extraction method? estimation method? HPLC column?

07 July 2013 6,117 3 View

Problem with the cDNA purity in RNA extraction by LiCl method

I have synthesized cDNA from RNA (extracted by LiCl method) using Thermo RevertAid cDNA synthesis kit. The 260/280 ratio for all the cDNA samples is falling in the range 1.6-1.7 while it should be...

06 June 2013 2,208 5 View