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Questions related from Ravinder Kumar
I am using HPLC for saponin estimation in plant samples. but the problem with separation of the close peaks. the HPLC conditions are.. 1. HPLC: Agilent 1260 infinity with DAD detector.2 2....
08 August 2017 3,623 23 View
i want to estimate the saponin content in crude extract of aloe vera using HPLC. which standard of saponin should i use to estimate the saponin or which type of saponin is found in Aloe vera?
03 March 2016 3,792 3 View
Suggest methos of total saponin estimation from Aloe vera. Also suggest the extration method and parameter of HPLC.
08 August 2015 9,011 7 View
I am using Biorad sequi gen sequencing gel apparatus for AFLP. In which one a plate is treated with Bind silane. After staining this is very difficult to remove the gel from the glass plate....
06 June 2014 4,993 4 View
We have sequenced the plant transcriptome using next generation sequencing. Now we have to Design SSR markers from this data. Please suggest how to design these markers.
04 April 2014 1,636 5 View
Seeking a suggestion of any DNA isolation method for different fungal strains. Some methods are working for particular strains. But I want a method which works for different strains and also best...
08 August 2013 3,376 1 View
Please suggest HPLC method for estimation of saponin. Extraction method? estimation method? HPLC column?
07 July 2013 6,119 3 View
I have synthesized cDNA from RNA (extracted by LiCl method) using Thermo RevertAid cDNA synthesis kit. The 260/280 ratio for all the cDNA samples is falling in the range 1.6-1.7 while it should be...
06 June 2013 2,209 5 View