Can I detect the chloroplast DNA in total genomic DNA without specific producer for chloroplast DNA extraction?

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Restriction enzymes with genomic DNA?

Hello, Is there anyone has any experience with using restriction enzymes with DNA before doing PCR? in case having a pure extracted DNA

07 August 2018 9,343 4 View

Can using the same rapd primers for fungi and plant ?

Can using the same primers to detect the variation in plant and fungi by RAPD PCR ?

07 August 2017 7,667 7 View

Aflatoxin genes I isolate ten A. flavus all of them gaves positive in Aflatoxin producing. while in PCR only 2 gene (omt and nor)?

I isolate ten A. flavus all of them gaves positive in Aflatoxin producing. while in PCR only 2 gene (omt and nor) were detected . no bands for apa and ver genes, can the fungus produce Aflatoxin...

04 May 2015 2,704 1 View

Can I detect the mtDNA in total genomic DNA without specific producer for mtDNA extraction?

09 October 2014 6,198 6 View

Why is there no DNA in a sample when I crashed the mycelium cells by liquid nitrogen and stored it until extraction?

08 September 2014 10,035 4 View

PCR Troubleshooting

Hi, have anyone have an electronic book about PCR Troubleshooting?

07 August 2014 2,568 2 View

What is the annealing temperature suitable for the PCR reaction?

I have primers, but the problem is they have different Tm with a big range. The forward is 70 the reverse is 63.

04 May 2014 6,907 23 View

What types of RNA should I extract for reverse transcriptase PCR, miRNA or large RNA (Total RNA)?

03 April 2014 3,256 8 View

Can anyone explain this "plant contains genes that homologous in structure and function to genes of MT in animal and fungi"?

Does this mean they have the same genes? If they are homologous, can I use the same PCR primers?

03 April 2014 5,819 3 View

Can I use the same PCR primers with different DNA organisms?

Sometimes when I am looking for some microorganisms primer genes in the web I found some of them, but have been used with different DNA organisms, can I use the same primers with different organisms?

02 March 2014 3,035 12 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View