PCR Troubleshooting

More Mohammad Ibrahim's questions See All

Restriction enzymes with genomic DNA?

Hello, Is there anyone has any experience with using restriction enzymes with DNA before doing PCR? in case having a pure extracted DNA

07 August 2018 9,343 4 View

Can using the same rapd primers for fungi and plant ?

Can using the same primers to detect the variation in plant and fungi by RAPD PCR ?

07 August 2017 7,667 7 View

Aflatoxin genes I isolate ten A. flavus all of them gaves positive in Aflatoxin producing. while in PCR only 2 gene (omt and nor)?

I isolate ten A. flavus all of them gaves positive in Aflatoxin producing. while in PCR only 2 gene (omt and nor) were detected . no bands for apa and ver genes, can the fungus produce Aflatoxin...

04 May 2015 2,704 1 View

Can I detect the mtDNA in total genomic DNA without specific producer for mtDNA extraction?

09 October 2014 6,198 6 View

Can I detect the chloroplast DNA in total genomic DNA without specific producer for chloroplast DNA extraction?

09 October 2014 1,026 2 View

Why is there no DNA in a sample when I crashed the mycelium cells by liquid nitrogen and stored it until extraction?

08 September 2014 10,035 4 View

What is the annealing temperature suitable for the PCR reaction?

I have primers, but the problem is they have different Tm with a big range. The forward is 70 the reverse is 63.

04 May 2014 6,907 23 View

What types of RNA should I extract for reverse transcriptase PCR, miRNA or large RNA (Total RNA)?

03 April 2014 3,256 8 View

Can anyone explain this "plant contains genes that homologous in structure and function to genes of MT in animal and fungi"?

Does this mean they have the same genes? If they are homologous, can I use the same PCR primers?

03 April 2014 5,819 3 View

Can I use the same PCR primers with different DNA organisms?

Sometimes when I am looking for some microorganisms primer genes in the web I found some of them, but have been used with different DNA organisms, can I use the same primers with different organisms?

02 March 2014 3,035 12 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Gromacs first step of minimization ?

I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...

07 August 2024 2,598 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you know any references for analyzing stochastic fiber orientaion composites ?

Hello everyone I am looking for one or some books for propertes and behaviors of stochastic fibre orientation composites. unfotunately I could not find any suitable reference for thias by...

04 August 2024 3,461 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View