PCR Troubleshooting

More Mohammad Ibrahim's questions See All

Restriction enzymes with genomic DNA?

Hello, Is there anyone has any experience with using restriction enzymes with DNA before doing PCR? in case having a pure extracted DNA

07 August 2018 9,264 4 View

Can using the same rapd primers for fungi and plant ?

07 August 2017 7,586 7 View

Aflatoxin genes I isolate ten A. flavus all of them gaves positive in Aflatoxin producing. while in PCR only 2 gene (omt and nor)?

I isolate ten A. flavus all of them gaves positive in Aflatoxin producing. while in PCR only 2 gene (omt and nor) were detected . no bands for apa and ver genes, can the fungus produce Aflatoxin...

04 May 2015 2,637 1 View

Can I detect the mtDNA in total genomic DNA without specific producer for mtDNA extraction?

09 October 2014 6,123 6 View

Can I detect the chloroplast DNA in total genomic DNA without specific producer for chloroplast DNA extraction?

09 October 2014 955 2 View

No title

08 September 2014 9,965 4 View

What is the annealing temperature suitable for the PCR reaction?

I have primers, but the problem is they have different Tm with a big range. The forward is 70 the reverse is 63.

04 May 2014 6,824 23 View

Can anyone explain this "plant contains genes that homologous in structure and function to genes of MT in animal and fungi"?

Does this mean they have the same genes? If they are homologous, can I use the same PCR primers?

03 April 2014 5,748 3 View

What types of RNA should I extract for reverse transcriptase PCR, miRNA or large RNA (Total RNA)?

03 April 2014 3,187 8 View

Can I use the same PCR primers with different DNA organisms?

Sometimes when I am looking for some microorganisms primer genes in the web I found some of them, but have been used with different DNA organisms, can I use the same primers with different organisms?

02 March 2014 2,946 12 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View