11 Questions 31 Answers 0 Followers
Questions related from Mohammad Ibrahim
Hello, Is there anyone has any experience with using restriction enzymes with DNA before doing PCR? in case having a pure extracted DNA
08 August 2018 9,319 4 View
Can using the same primers to detect the variation in plant and fungi by RAPD PCR ?
08 August 2017 7,643 7 View
I isolate ten A. flavus all of them gaves positive in Aflatoxin producing. while in PCR only 2 gene (omt and nor) were detected . no bands for apa and ver genes, can the fungus produce Aflatoxin...
05 May 2015 2,689 1 View
Can I detect the chloroplast DNA in total genomic DNA without specific producer for chloroplast DNA extraction?
10 October 2014 1,004 2 View
Can I detect the mtDNA in total genomic DNA without specific producer for mtDNA extraction?
10 October 2014 6,176 6 View
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09 September 2014 10,013 4 View
Hi, have anyone have an electronic book about PCR Troubleshooting?
08 August 2014 2,538 2 View
I have primers, but the problem is they have different Tm with a big range. The forward is 70 the reverse is 63.
05 May 2014 6,880 23 View
Does this mean they have the same genes? If they are homologous, can I use the same PCR primers?
04 April 2014 5,800 3 View
What types of RNA should I extract for reverse transcriptase PCR, miRNA or large RNA (Total RNA)?
04 April 2014 3,237 8 View
Sometimes when I am looking for some microorganisms primer genes in the web I found some of them, but have been used with different DNA organisms, can I use the same primers with different organisms?
03 March 2014 3,010 12 View
