Problem with PCR using plasmid as template?

Hi I am trying to do a PCR for a 1kb fragment using a plasmid of 11kb as a template. But unfortunately on the gel, the band is as large as the plasmid, that is 11kb. Would anyone know that that...

20 October 2020 7,631 3 View

How to fetch data of a .sslist file?

We are using Mauve software for alignment of genomes of nematodes having genomic size approximately 100 Mb. One of the output file generated using this software has extension .sslist file. Unable...

24 September 2020 7,520 1 View

How the saturation temperature is to be provided in evaporation condensation model in ANSYS FLUENT?

I am planning to simulate condensation of moist air in ANSYS FLUENT. Say the inlet conditions of air is at 30 degree Celsius and relative humidity 80%. While giving mass transfer mechanism in...

09 August 2020 7,744 3 View

How to give relative humidity input in ANSYS FLUENT?

I am trying to simulate the condensation of moist air in ANSYS FLUENT. Say, the inlet temperature of air is 30 degree Celsius with relative humidity 80%. I assume that species transport model is...

03 August 2020 7,778 2 View

How to give relative humidity input in ANSYS FLUENT?

I am trying to simulate the condensation of moist air in ANSYS FLUENT. Say, the inlet temperature of air is 30 degree Celsius with relative humidity 80%. I assume that species transport model is...

03 August 2020 211 3 View

Can anyone suggest a way to overcome recombination when amplifying a pcDNA3 plasmid containing human SCN9A CDS insert?

I need to amplify a pcDNA3 plasmid containing human SCN9A CDS insert (pcDNA3-SCN9A construct). The CDS is around 6 kb, and the total construct length is approximately 11.2 kb, which is a bit...

07 June 2020 2,134 2 View

How can i extract RCM Data corresponding to observation station using GIS?

I have downloaded CORDEX SA data corresponding to the variables such as temperature and precipitation. I also have ground observation data for the above variables. i want to extract CORDEX SA data...

02 April 2020 7,757 7 View

Can anyone suggest me the best suited shape for quantum well based LED device?

Among different shapes of well, which is the most suited well in terms of efficiency

18 September 2019 7,798 1 View

What does that mean when the standard deviation is presented in percentage in a research paper?

"Sample size was calculated on the basis of the primary endpoint, assuming a SD of 20%, a 2-sided type I error of 5%, a power of 80%, and a difference of 20 points in percentage change from...

18 August 2019 2,612 3 View

How elementary particles acquire mass and charge?

Elementary particles have their own mass and charge. Mass is known to be acquired by the Higgs field. How charge is acquired by a particle?. Is both the phenomena are dependent?.

08 June 2019 9,722 13 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View