What is the mechanism in which BSA does so? also, I have seen many protocols for cell fixation/permeabilization where they don't use any stabilizer and just a few where BSA is used with a buffer for stabilization. Is BSA really needed? as I understand, the fixation stops any metabolic activity, so why should I use a stabilizer?
Thank you
One reason would be to prevent proteins from sticking to the walls of tubes and to pipet tips by blocking any non-specific binding
Hi Govinda, thank you for your answer, but do you know what happens from the molecular point of view? how is this blocking and why is BSA inert?
Hello Vanessa,
I think that you can find (partial?) answers from big sellers of the products. As an example:
http://www.sigmaaldrich.com/catalog/product/sigma/a8412?lang=fr®ion=BE
Best,
Jacques
Hi, I don't know if this still useful to you.
https://www.thermofisher.com/mx/es/home/life-science/cell-analysis/cell-analysis-learning-center/molecular-probes-school-of-fluorescence/imaging-basics/sample-considerations/preparing-fixed-cells-imaging.html
Hi, I don't know if this still useful to you.
https://www.thermofisher.com/mx/es/home/life-science/cell-analysis/cell-analysis-learning-center/molecular-probes-school-of-fluorescence/imaging-basics/sample-considerations/preparing-fixed-cells-imaging.html
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