Can catalase fluoresce at Ex/Em ~ 310/420nm?

Catalase has intrinsic fluorescence due to its tryptophan and tyrosine residues, but I wonder if it can fluoresce at a longer wavelength, due to other residues (such as NADPH). I haven't seen...

03 April 2018 9,629 3 View

Could somebody explain how NADPH (or NADPH-enzyme complex) can be secreted by cells?

I'm working with gram negative bacteria, and I would like to know how NADPH could be excreted, or if it is something related to cells lysis. Thank you,

01 February 2018 6,263 0 View

CDCFDA emission in dead cells?

I treated pertussis cells with ethanol and then I analyzed them in a flow cytometer after staining with cDCFDA. I would like to know why I'm getting such a strong fluorescence signal with cDCFDA...

08 September 2017 647 3 View

DCFDA for ROS detection?

When I worked with DCFDA in B. pertussis, sometimes I see a yellow color after one hour of incubation (protected from light at room temperature) and sometimes not. For carboxy-DCFDA, I don't see...

04 May 2017 4,131 5 View

DCFDA reaction with H2O2?

Could somebody please explain why I'm obtaining fluorescence signal when I combine DCFDA and H2O2 in buffer? My understanding is that DCFDA must first react with esterases to eliminate the acetyl...

01 February 2017 5,925 3 View

Degradation of DNA and RNA in bacteria?

I understand that degradation of DNA doesn't happen so quickly as RNA. Somebody could please explain why and how this can affect flow cytometric analysis? I haven't seen any protocol for dyes that...

06 July 2016 1,939 0 View

Doublets discrimination in flow cytometry?

The flow cytometer that I'm using just have the option of width and area for the FL2 detector (yelow/orange fluorescence), but I'm just using the green and the red detectors (FL1 and FL3). For...

02 March 2016 8,104 5 View

Why is BSA used to stabilize solutions?

What is the mechanism in which BSA does so? also, I have seen many protocols for cell fixation/permeabilization where they don't use any stabilizer and just a few where BSA is used with a buffer...

02 March 2016 8,377 6 View

What is the number of events in flow cytometry ?

As I understand the events are the numbers of cells passing through the laser. So, why are there cell counting beads for quantification of cells? having the number the events is not enough? what...

31 December 2015 6,098 7 View

For flow cytometry gating, what is the difference in doing the gating in the flow cytometer and doing it in the software?

When I do the gating in the flow cytometer, does it just record the part that is in the gate? could I do the run without gating in the piece of equipment and then do that in the software?

31 December 2015 6,011 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View