Govinda Sharma

7 Questions 127 Answers 0 Followers

Questions related from Govinda Sharma

Have you ever used cytotoxic T-cells in a cytotoxicity assay (eg. Cr51-release or something equivalent) directly after thawing them?

I have aliquots of expanded cytotoxic T-cells that I would like to use directly in a cytoxicity assay after thawing the cells without re-stimulating them. So far I've tried thawing them, resting...

10 October 2017 8,790 3 View

ThermoFisher Mouse T-activator Dynabeads suddenly stopped working for me. Has anybody else had a similar problem?

Hi all, I had been using ThermoFisher's mouse T-activator kit (Catalog number: 11456D) to expand CD8 T-cells from mouse splenocytes. For around 2 years I had great results, got 200-fold expansion...

02 February 2017 1,526 2 View

U-bottom plates in Tecan Safire2?

HI all, I'm interested in using the Tecan Safire2 to do an emission scanning experiment on T-cell cultures that I am treating in U-bottom 96-well plates. I've noticed that all the default plate...

04 April 2016 7,531 0 View

My EL4 cells had a massive die-off. Any idea why?

I've been working with the mouse thymoma line, EL4, and have had a couple occasions in which the cells have suddenly and completely died off. Is it known if these cells have a senescence limit...

01 January 2016 8,717 4 View

Which automated cell counter is best for handling small cells such as splenocytes and thymocytes (~8 micron diameter)?

We have an Invitrogen Countess counter which works really well for most mammalian cell lines. However, it seems to be less accurate when enumerating small cells - especially with respect to their...

01 January 2016 8,926 7 View

Does anybody know the proportion of Ova-specific OT-I T-cells from the OT-I mouse express a second TCR from rearrangement of the endogenous locus?

The natural TCR locus of this TCR transgenic mouse is still intact and is capable of rearranging. Is there any data out there regarding the frequency at which other productive TCRs are...

06 June 2015 4,413 2 View

Is the compensation value set in a flow cytometry experiment dependent on the brightness of the fluorophore?

If I've got a compensation control that is much brighter than my experimental sample, is the compensation value derived from this control still valid? Intuitively, I feel that more fluorescence...

11 November 2014 1,354 5 View